gms | German Medical Science

61st Annual Meeting of the German Society of Neurosurgery (DGNC) as part of the Neurowoche 2010
Joint Meeting with the Brazilian Society of Neurosurgery on the 20 September 2010

German Society of Neurosurgery (DGNC)

21 - 25 September 2010, Mannheim

Attenuation of cell motility and invasion by treatment of U87 glioma cells with the nitric oxide donor JS-K in vitro

Meeting Abstract

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  • Stefanie Bette - Abteilung Allgemeine Neurochirurgie, Universit酹sklinikum Freiburg, Germany
  • Brunhilde Baumer - Abteilung Allgemeine Neurochirurgie, Universit酹sklinikum Freiburg, Germany
  • Astrid Weyerbrock - Abteilung Allgemeine Neurochirurgie, Universit酹sklinikum Freiburg, Germany

Deutsche Gesellschaft fr Neurochirurgie. 61. Jahrestagung der Deutschen Gesellschaft fr Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010. Mannheim, 21.-25.09.2010. Dsseldorf: German Medical Science GMS Publishing House; 2010. DocP1724

doi: 10.3205/10dgnc195, urn:nbn:de:0183-10dgnc1950

Published: September 16, 2010

© 2010 Bette et al.
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Outline

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Objective: The diazeniumdiolate JS-K generates nitric oxide (NO) on enzymatic activation in GST-expressing cells. In addition to growth-inhibitory and chemosensitizing effects in tumors including U87 gliomas, JS-K has anti-invasive effects in breast cancer cells. The objective was to evaluate the anti-migratory effect of JS-K in U87 glioma cells in vitro.

Methods: Migration and invasion were assessed using cell motility and Boyden chamber assays. U87 cells were grown to confluence in 6-well plates separated by silicone walls. After removal of the walls they were treated with JS-K concentrations between 1–10然. The distance between cell populations was measured after 6, 24, and 48h using Axiovision LE-software (Zeiss). To study invasion, 105 U87 cells were plated on inserts with 8痠 pore-size polycarbonate filters coated with Matrigel (0,7mg/ml) and incubated for 24hours with JS-K (1–10然). Cell migration was evaluated by counting five fields on each filter and expressed as the mean number of cells that had migrated through the filter. The experiments were repeated at least three times and analysed by ANOVA.

Results: JS-K significantly reduced the capacity of U87 cells to migrate towards each other and form a confluent monolayer in a cell motility assay at doses which did not affect viability. Migration across a matrigel-coated membrane was also significantly attenuated in a concentration-dependent manner at 5然 and 10然, but not at 1 然 (p=0.0009 and p<0.0001, respectively).

Conclusions: This is the first report that the NO donor JS-K has anti-migratory effedtsoin U87 glioma cells in vitro. Effects on proliferation and migration and chemosensitizing properties make NO donors good candidates for multimodal cancer therapy.