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61st Annual Meeting of the German Society of Neurosurgery (DGNC) as part of the Neurowoche 2010
Joint Meeting with the Brazilian Society of Neurosurgery on the 20 September 2010

German Society of Neurosurgery (DGNC)

21 - 25 September 2010, Mannheim

Neutrophils commonly penetrate the glioblastoma venule wall

Meeting Abstract

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  • Richard E. Kast - Department of Psychiatry University of Vermont, Burlington, VT, USA
  • Marc-Eric Halatsch - Klinik für Neurochirurgie, Universitätsklinikum Ulm, Germany

Deutsche Gesellschaft für Neurochirurgie. 61. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010. Mannheim, 21.-25.09.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. DocP1714

doi: 10.3205/10dgnc185, urn:nbn:de:0183-10dgnc1855

Published: September 16, 2010

© 2010 Kast et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Objective: Desbaillets et al in 1997, and Fossati et al. in 1999 and Iwatsuki et al. in 2000 showed neutrophil infiltration in glioblastomas. We wanted to confirm these observations to better understand the role of neutrophils in glioblastoma growth.

Methods: Seven glioblastoma biopsies were dually stained with H & E and an esterase stain that preferentially stains neutrophils. The entire slide was examined for neutrophils by light microscopy and micrographs were taken with a CCD camera. Areas of necrosis were avoided and not scored.

Results: Thirty-five percent of the venules had neutrophils either adherant to venule luminal wall or in various stages of penetration of the wall. Of these venules with neutrophils, intramural degranulation was common and in 11% of these venules degranulation was seen within the vessel lumen.

Conclusions: We confirm the presence of neutrophils within glioblastoma tissue but could not confirm Fossati et al. and Iwatsuki et al.'s observation of neutrophils preferentially in areas of penetration into normal brain. We saw neutrophils preferentially penetrating venules. Therapeutic implications of these findings are discussed.