gms | German Medical Science

59th Annual Meeting of the German Society of Neurosurgery (DGNC)
3rd Joint Meeting with the Italian Neurosurgical Society (SINch)

German Society of Neurosurgery (DGNC)

1 - 4 June 2008, Würzburg

Detection of inactivated tumour suppressor genes in meningiomas of different WHO grades by methylation-specific multiplex ligation-dependent probe amplification – first results

Erfassung inaktivierter Tumorsuppressor Gene in Meningiomen unterschiedlichen WHO Grades mit der methylierungsspezifischen multiplex-ligation dependent probe amplification – erste Ergebnisse

Meeting Abstract

  • corresponding author C. Ewald - Klinik für Neurochirurgie, Klinikum der Friedrich Schiller Universität, Jena, Germany
  • S. A. Kuhn - Klinik für Neurochirurgie, Klinikum der Friedrich Schiller Universität, Jena, Germany
  • C. Beetz - Institut für klinische Chemie und Laboratoriumsdiagnostik, Klinikum der Friedrich Schiller Universität, Jena, Germany
  • T. Deufel - Institut für klinische Chemie und Laboratoriumsdiagnostik, Klinikum der Friedrich Schiller Universität, Jena, Germany
  • R. Kalff - Klinik für Neurochirurgie, Klinikum der Friedrich Schiller Universität, Jena, Germany

Deutsche Gesellschaft für Neurochirurgie. Società Italiana di Neurochirurgia. 59. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3. Joint Meeting mit der Italienischen Gesellschaft für Neurochirurgie (SINch). Würzburg, 01.-04.06.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. DocP 004

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2008/08dgnc272.shtml

Published: May 30, 2008

© 2008 Ewald et al.
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Outline

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Objective: Inactivation of tumour suppressor genes (TSGs) has been detected in several cerebral tumours during the last years with some of them becoming more and more relevant for special treatment. Concerning meningiomas the genetical changes leading to tumour recurrence and / or tumour progress still remain unclear. We describe now our first experiences with 15 meningioma specimen analysed by methylation specific multiplex ligation dependent probe amplification (MS – MLPA) as a relatively simple approach to detect inactivated TSGs.

Methods: 37 known tumour suppressor genes on 40 different gene loci were scanned in a series of 15 intracranial meningiomas of different biological behaviour (Grade I n = 9, II = 4, III = 2) promoter methylation and deletion/amplification status by MS – MLPA.

Results: The most frequent events in meningiomas were deletions on 22q (10/15), 12q (10/15), 11q (11/15), 3p (12/15) and 1p (10/15) as well as amplifications on 6q (12/15). This confirms previous findings (e.g. 22q) but also suggests novel frequently affected regions (e.g. 12q or 11q). In general the number of aberrations tended to be higher in more aggressive tumours like grade III meningiomas. The VHL tumour suppressor gene on 3p was the most affected one (12/15) followed by GSTP1 and TP73. Promoter methylation was only observed for the RASSF1 gene in one grade II tumour.

Conclusions: Our technique seems to be a robust, reliable and simple method to detect gene function loss caused by deletion, amplification and/or promoter hypermethylation in intracranial tumour tissue providing a better understanding of tumour genesis and malignisation in intracranial meningiomas. We could confirm previous findings as well as we can present possible new genetical features contributing to tumour growth and malignisation.