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58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

26. bis 29.04.2007, Leipzig

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The influence of blood on the development of edema and the size of the lesion in acute subdural haematoma in the rat

Der Einfluss des Blutes bei akuten Subduralhämatomen auf die Oedementwicklung und Größe der Läsion im Rattenhirn

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  • corresponding author Heidrun Bächli - Neurochirurgische Klinik, Universitätskrankenhaus und Universitäts-Kinderkrankenhaus, Basel (UKBB)
  • S. Ens - Institut für Neurochirurgische Pathophysiologie, Johannes Gutenberg-Universität Mainz, Deutschland
  • M. Behzad - Institut für Neurochirurgische Pathophysiologie, Johannes Gutenberg-Universität Mainz, Deutschland
  • O. Kempski - Institut für Neurochirurgische Pathophysiologie, Johannes Gutenberg-Universität Mainz, Deutschland
  • B. Alessandri - Institut für Neurochirurgische Pathophysiologie, Johannes Gutenberg-Universität Mainz, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC). Leipzig, 26.-29.04.2007. Düsseldorf: German Medical Science GMS Publishing House; 2007. DocFR.03.03

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2007/07dgnc077.shtml

Published: April 11, 2007

© 2007 Bächli et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Objective: Acute subdural haematoma (ASDH) has a poor prognosis and high mortality rate (60-90%) even with prompt surgical treatment. The purpose of this study was to analyze the role of blood and volume in the development of secondary brain damage.

Methods: Male Sprangue-Dawley rats (n=49, weight 300-350g) were divided in 3 groups: blood (n=20), paraffin oil (n=17) and sham operated (n=12). Anaesthetic/medication: chloral hydrate i.p. initially 1ml/100g, afterwards 1ml/h; 1ml atropine 1mg s.c. Operation: A venous catheter (femoral or jugular vein) collected blood (300ml). Through a burr hole (1mm), the dura was opened and a bent L-shaped cannula (G23) introduced and fixed with Histoacryl. An ICP probe (Raumedic) was inserted contralaterally. ASDH: introduction of 300ml unheparinised blood or paraffin oil with a flow rate of 50ml/min., sham no infusion. Measurement of edema by the wet-dry weight method. Staining with haematoxylin-eosin enabled the measurement of the lesion volume.

Results: ICP values were not statistically different in the two infusion groups. Animals with subdurally injected blood had a significantly higher water content (ipsilateral hemisphere 80.96±0.55%, contralateral 79.04±0.10%) than the paraffin group (ipsi 79.20±0.10, contra 78.61±0.09%) and sham (ipsi 78.69±0.15%, contra 78.58±0.11%). The lesion volume was also larger in the blood group (blood 40.15±8.40mm3; paraffin 7.34±3.13mm3 and sham 0.50±0.07mm3).

Conclusions: The haematoma itself, not the extravasal volume of liquid, seems to play the decisive role in the extent of secondary brain damage after ASDH.