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56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

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Tumor-produced extracellular matrix influences brain tumor tropism of human neural stem cells

Tumor produzierte extrazelluläre Matrix beeinflusst den Hirntumor-Tropismus menschlicher neuraler Stammzellen

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  • corresponding author N. O. Schmidt - Neurosurgical Oncology Laboratory, Brigham & Women's Hospital/Harvard Medical School, Boston, USA; Klinik für Neurochirurgie, Universitätsklinikum HH-Eppendorf, Hamburg
  • M. Ziu - Neurosurgical Oncology Laboratory, Brigham & Women's Hospital/Harvard Medical School, Boston, USA
  • K. Aboody - City of Hope Cancer Center & Beckman Research Institute, Duarte, USA
  • M. Westphal - Klinik für Neurochirurgie, Universitätsklinikum HH-Eppendorf, Hamburg
  • P. M. Black - Neurosurgical Oncology Laboratory, Brigham & Women's Hospital/Harvard Medical School, Boston, USA
  • R. S. Carroll - Neurosurgical Oncology Laboratory, Brigham & Women's Hospital/Harvard Medical School, Boston, USA

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP174

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2005/05dgnc0442.shtml

Published: May 4, 2005

© 2005 Schmidt et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

A major obstacle in the treatment of gliomas is the invasive capacity of the tumor cells. Previous studies have demonstrated the capability of neural stem cells (NSCs) to target infiltrated tumor cells and to deliver therapeutic gene products. However, the signals involved in the brain tumor tropism of NSCs and their interactions within the tumor environment are hardly defined.

Methods

As gliomas progress and invade, an extensive modulation of the extracellular matrix (ECM) occurs. Tumor-ECM derived from 6 glioblastoma cell lines and purified ECM compounds known to be upregulated in the glioma environment were analyzed for their effects on NSCs motility in vitro.

Results

We found that tumor-produced ECM was highly permissive for NSC migration. Laminin was the most permissive substrate for human NSCs migration, and tenascin-C the strongest inducer of a directed human NSC migration (haptotaxis). A positive correlation between the degree of adhesion and migration of NSCs on different ECM compounds exists, as for glioma cells.

Conclusions

Our data indicate that the ECM of malignant gliomas is a significant modulator of NSC migration. ECM proteins preferentially expressed in areas of glioma cell invasion may serve as additional local guidance signal for NSC tropism to disseminated tumor cells.