gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

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Specific intensity imaging for glioblastoma and neural cell cultures with 5-aminolevulinic acid-derived protoporphyrin IX

Visualisierung von Glioblastomzelllinien und neuralen Zellkulturen mit durch 5-Aminolevulinsäure induziertem Protoporphyrin IX

Meeting Abstract

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  • corresponding author R. Ritz - Eberhard-Karls-Universität, Klinik für Neurochirurgie, Tübingen
  • F. Roser - Eberhard-Karls-Universität, Klinik für Neurochirurgie, Tübingen
  • D. Freudenstein - Eberhard-Karls-Universität, Klinik für Neurochirurgie, Tübingen
  • F. Duffner - Eberhard-Karls-Universität, Klinik für Neurochirurgie, Tübingen
  • M. Tatagiba - Eberhard-Karls-Universität, Klinik für Neurochirurgie, Tübingen

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP161

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2005/05dgnc0429.shtml

Published: May 4, 2005

© 2005 Ritz et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

The fluorescence of protophorphyrin IX (PpIX) synthesized after incubation with 5-aminolevulinic acid (5-Ala) is used in vivo for intraoperative visualisation of gliomas. A further useful application may be photodynamic therapy (PTD) for local glioma treatment. As a basic requirement for this application, the difference in the fluorescence intensity of tumor cells compared to neurons should be significant.

Methods

To test this hypothesis, eight different human glioma cell lines (LN-18, LN-428, U87MG, U373MG, D247MG, U251MG, LN-308, T98G) were compared with transformed human astrocytes and rat neurons after incubation for different periods of time in vitro with 5-Ala [1 mg/ml]. Fluorescence intensity profiles were measured by a digital camera comparing glioma cell lines with control cells.

Results

All glioma cell lines could be distinguished from neural cells by the intensity of their fluorescence. This was proven by post hoc tests for pairwise comparisons using Tukey's honestly significant difference test, at the global significance level of 5%.

Conclusions

Various glioma cell lines showed different fluorescence intensities, therefore limiting the method.