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55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

25. bis 28.04.2004, Köln

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Effect of intraischemic arterial normobaric hyperoxia on neurobehavioral deficits in a focal mass lesion model in rodents

Auswirkung einer intraischämischen, normobaren, arteriellen Hyperoxie auf das Verhaltensmuster nach fokaler, epiduraler Läsion im Rattenmodell

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  • corresponding author Ralf Burger - Neurochirurgische Klinik, University of Regensburg, Regensburg
  • E.-M. Stoerr - Neurochirurgische Klinik, University of Regensburg, Regensburg
  • T. Finkenzeller - Radiology, University of Regensburg, Regensburg
  • U. Dorenbeck - Radiology, University of Regensburg, Regensburg
  • A. Brawanski - Neurochirurgische Klinik, University of Regensburg, Regensburg

Deutsche Gesellschaft für Neurochirurgie. Ungarische Gesellschaft für Neurochirurgie. 55. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 1. Joint Meeting mit der Ungarischen Gesellschaft für Neurochirurgie. Köln, 25.-28.04.2004. Düsseldorf, Köln: German Medical Science; 2004. DocP 01.7

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2004/04dgnc0290.shtml

Published: April 23, 2004

© 2004 Burger et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective

The effect of an intraischemic arterial normobaric hyperoxia on a 2 week assessment of behavorial deficits was evaluated in a focal mass lesion model in rodents.

Methods

Fifty Spraque Dawley rats were included (244 ± 36g) in this study and neurobehavioral testing was possible in 33/ 50 animals. Baseline measurement (59 ± 6 min) of brain partial oxygen pressure [p(ti)O2] and EEG was followed by induction of an epidural focal mass lesion with a balloon. The endpoint of balloon inflation was defined by flattening of the EEG and the mass lesion was prolonged over 60 min. After reperfusion, study groups were followed over 61 ± 4 min before animals were extubated and brought back to their cages. Neurobehavior was assessed over 14 days [beam balance (BB); beam walking (BW); open field (OF); postural reflex (PR); Morris water maze (MWM)]. Study groups with arterial normoxia (paO2~100 mmHg, n = 25) during prolonged balloon inflation and intraischemic arterial, normobaric hyperoxia (paO2 ~ 250 mmHg, n = 13) were compared to non – lesioned, sham – operated animals (n = 12). The lesion was also evaluated by MRI and histology.

Results

Sham – operated animals showed a normal EEG pattern, p(ti)O2 values in the normal range and a regular neurobehavior over time. All injured animals showed a significant worsening of the EEG over time course (p < 0.05) but no differences between the groups (p = 0.162). Intraischemic P(ti)O2 increased to normal values due to normobaric hyperoxia, while normoxic animals showed ischemic values (p<0.001). Neurobehavior in animals with a lesion showed a significant worsening of test results (BB p<0.05, BW p<0.05, PR p<0.05, MWM p<0.05) compared to sham operated animals. Neurobehavioral deficits of animals treated with intraischemic hyperoxia were equal (OF) or more pronounced (BW, BB, PR and WMT) compared to animals with arterial normoxia.

Conclusions

Intraischemic induction of arterial hyperoxia to increase oxygen supply and brain p(ti)O2 induced worsening of neurobehavioral outcome after a focal mass lesion.