Article
Tissue arrays have shown different immunohistological expression of both target genes EMMRPIN and MMP2 in primary lung tumors and their central nervous metastasis within the same patients – By the way an economic tool reducing laboratory costs
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Authors
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Ulrich Zils
- HELIOS-Klinikum Berlin-Buch, Klinik für Neurochirurgie, Berlin
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Ali-Fuat Okuducu
- Klinikum Nürnberg, Institut für Pathologie, Nürnberg
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Jan Reinhardt
- Schweizer Paraplegiker Forschung/Universität Luzern, Koordination Forschung/Universität Luzern, Nottwil/Luzern
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Gunda Leschber
- Evangelische Lungenklinik Berlin - Krankenhausbetriebs gGmbH, Thoraxchirurgische Klinik, Berlin
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Frank Schäper
- Evangelische Lungenklinik Berlin - Krankenhausbetriebs gGmbH, Institut für Pathologie, Berlin
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Farid Youssef
- HELIOS-Klinikum Berlin-Buch, Klinik für Neurochirurgie, Berlin
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Asaad Sharafelddin
- HELIOS-Klinikum Berlin-Buch, Klinik für Neurochirurgie, Berlin
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Jürgen Kiwit
- HELIOS-Klinikum Berlin-Buch, Klinik für Neurochirurgie, Berlin
| Published: | April 23, 2012 |
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Outline
Text
Introduction: To elucidate a real possible key role of target genes in lung-tumorprogression and cerebral metastasis we created special tissue arrays of tumor samples and their cerebral metastasis of one and the same patients embedded in one and the same paraffin block. Due to the dominant role of Matrixmetalloproteases (MMPs) for metastasis and due to the most prominent lung cancer in the western world we first decided to investigate lung tumors and the matrixmetalloproteinase system. We investigated the expression of EMMPRIN (extracellular matrix metalloprotease inducer) and MMP 2 in brain metastasis of lung tumors and their primary tumor in the same patient.
Materials and methods: Data were collected from 12 patients with 17 metastases. Special tissue arrays were performed with representative areas of primary tumors and their cerebral filiae. Three stained slides were necessary instead of 29 stainings only. semiquantitative analysis was performed according to the method described by Remmele et al.. Following outcomes were of interest: EMMPRIN (cytoplasma staining) in percent, EMMPRIN intensity, EMMPRIN cytoplasma staining score, MMP2 (cytoplasma staining) in percent, MMP2 intensity, MMP2 cytoplasma staining score. Wilcoxon-tests for paired samples were run for analysis.
Results: Primary tumors showed lower outcomes scores than the filia tumors. Significance was given at the 5% level for EMMPRIN ZP in percent, EMMPRIN intensity, EMMPRIN ZP score, MMP2 ZP in percent, and MMP2 ZP score. No significance was given for MMP2 intensity. We used three new created paraffin embedded blocks instead of 29 separated ones only. Therefore 10 percent of the normal amount of antibody-sample was necessary to reach the same answer.
Conclusion: Tissue arrays have shown evidence for dedifferentiation of tumor tissue despite therapy. Primary tumor samples express a lower amount of EMMPRIN and MMP2 then their metastases. This could be demonstrated by using 10 percent of the conservative amount of antibody-samples only.
