gms | German Medical Science

128. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

03.05. - 06.05.2011, München

Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies

Meeting Abstract

  • Vilma Frick - Allgemeine Chirurgie, Allgemein-, Viszeral-, Gefäß-, und Kinderchirurgie, Homburg/Saar
  • Claudia Rubie - Allgemeine Chirurgie, Allgemein-, Viszeral-, Gefäß-, und Kinderchirurgie, Homburg/Saar
  • Pirus Ghadjar - Inselspital, Universität Bern , Radio-Onkologie, Bern
  • Martin K. Schilling - Allgemeine Chirurgie, Allgemein-, Viszeral-, Gefäß-, und Kinderchirurgie, Homburg a.d. Saar

Deutsche Gesellschaft für Chirurgie. 128. Kongress der Deutschen Gesellschaft für Chirurgie. München, 03.-06.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgch763

DOI: 10.3205/11dgch763, URN: urn:nbn:de:0183-11dgch7630

Published: May 20, 2011

© 2011 Frick et al.
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Outline

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Introduction: Chemokines have been proposed to contribute to tumour growth and metastatic spread of several cancer entities. Here, we examined the relative levels of CXCL12/CXCR4 in resection specimens from patients with different malignant and non-malignant colorectal diseases as well as colorectal liver metastases (CRLM).

Materials and methods: CXCL12/CXCR4 mRNA and protein expression profiles were assessed by quantitative real-time PCR (Q-RT-PCR), Western blot analysis, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in resection specimens from patients with ulcerative colitis (UC, n=15), colorectal adenoma (CRA, n=15), colorectal adenocarcinoma (CRC, n=47) and CRLM (n=16). Corresponding non-affected tissues served as control.

Results: In contrast to UC tissues, CXCL12 showed a distinct down-regulation in CRA, CRC and CRLM specimens, whereas the corresponding receptor CXCR4 demonstrated a significant up-regulation in CRC and CRLM related to corresponding non-affected tissues (p<0.05, respectively).

Conclusion: Our results strongly suggest an association between CXCL12/CXCR4 expression and the induction of CRA, CRC and the development of CRLM. Therefore, CXCR4 may be a potential target for specific therapeutic interventions.