gms | German Medical Science

128. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

03.05. - 06.05.2011, München

The role of IL-10 and Rho-Inhibition in nerve regeneration in an end-to-side nerve repair model

Meeting Abstract

  • Maria Sakalidou - Universitätsklinikum Freiburg, Plastische und Handchirurgie, Freiburg
  • G. Björn Stark - Universitätsklinikum Freiburg, Plastische und Handchirurgie, Freiburg
  • Klaus Aktories - Albert-Ludwigs Universität Freiburg, Freiburg
  • Vincenzo Penna - Universitätsklinikum Freiburg, Plastische und Handchirurgie, Freiburg

Deutsche Gesellschaft für Chirurgie. 128. Kongress der Deutschen Gesellschaft für Chirurgie. München, 03.-06.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgch650

DOI: 10.3205/11dgch650, URN: urn:nbn:de:0183-11dgch6508

Published: May 20, 2011

© 2011 Sakalidou et al.
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Outline

Text

Introduction: The end-to-side model is an established model used in clinical practice and experimental research. In end-to-side neurorrhaphy, nerve fiber regeneration occurs along the distal stump of a transected nerve by inducing collateral sprouting from a healthy donor nerve. This model, in which nerve regeneration does not reach the level of that of an end-to-end model is suitable to show differences between experimental groups. The purpose of this study was to investigate the role of IL-10 and C3 Toxin in nerve regeneration using an end-to-side nerve repair model.

Materials and methods: Thirty rats were used divided into 3 groups: (1) Control group, end-to-side nerve repair of the peroneal nerve onto the tibial nerve; (2) intrafasciculary injection of 0,125 μg/100 µl IL-10; or (3) 1 µg/100μl C3 fusion toxin into the repair site. After 8 weeks, the outcome was assessed. Motor function of the nerves was evaluated using the walking track test and calculating the Peroneal Functional Index (P.F.I.). For electrophysiological evaluation, the nerve conduction velocities (NCVs) were analyzed and histomorphological evaluation consisted of measurement of the collagen levels using picrosirius red staining and evaluation of myelination using methylene blue staining.

Results: There weren’t any statistical significant differences in the P.F.I. and NCV measurements. Histologic studies revealed a thicker myelin sheath and a lower G-ratio in the IL-10 group, indicating a better myelination with differences being respectively statistically significant among all groups (p<0.001). In the C3 Toxin group, a significant higher number of axons compared to the other two groups was found (p<0.05). Morphologic analysis demonstrated significant lower collagen levels in the IL-10 group (p<0.001), suggesting lower scar formation.

Conclusion: These results suggest that a low dose of 0,125 μg/ 100 µl IL-10 has a favorable effect in nerve regeneration in an end-to-side neurorrhaphy and reduces scar formation. This finding could help enhance clinical nerve surgery.