gms | German Medical Science

128. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

03.05. - 06.05.2011, München

The miRNAome of locally advanced rectal cancer

Meeting Abstract

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  • Jochen Gaedcke - Universitätsmedizin Göttingen, Allgemein- und Viszeralchirurgie, Göttingen

Deutsche Gesellschaft für Chirurgie. 128. Kongress der Deutschen Gesellschaft für Chirurgie. München, 03.-06.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgch335

DOI: 10.3205/11dgch335, URN: urn:nbn:de:0183-11dgch3354

Published: May 20, 2011

© 2011 Gaedcke.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

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Introduction: Increasing evidence suggests that microRNAs play a key role in the initiation and progression of cancer, and, therefore, may comprise a novel class of molecular biomarkers with prognostic and predictive potential. For colon cancer, several miRNA profiles have been published, however, data about miRNA regulation in rectal cancer are rare.

Materials and methods: RNA was extracted from tumor biopsies and normal adjacent mucosa from 72 patients with locally advanced rectal cancer). Each sample’s microRNA expression pattern was assayed on an LNA (Locked Nucleic Acid) enhanced microarray platform. To assess the relevance of differentially expressed miRNAs as blood-based biomarker, certain miRNAs were isolated from the plasma of 35 rectal cancer patients and 10 healthy controls using a modified Trizol protocol.

Results: More than 200 miRNAs were differentially expressed between rectal cancer and normal adjacent tissue (p<10-6). Some of the most significantly deregulated miRNAs were miR-143, miR-145, miR-21, miR-223, and members of the let-7 family, which have previously been reported to be specific for colon cancer. We also identified a subset of miRNAs that appear to be specific to rectal cancer. Out of 15 miRNA that were specifically upregulated in the tumor, none proved to be a blood-based biomarker for rectal cancer.

Conclusion: This study, for the first time, demonstrates a rectal cancer specific microRNA profile.