gms | German Medical Science

127. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

20.04. - 23.04.2010, Berlin

Renoprotective effects of progenitor cell therapy in renal ischemia-reperfusion injury: decompression required

Meeting Abstract

  • Tanja Herrler - Klinikum Großhadern, LMU München, Chirurgische Klinik, München, Deutschland
  • Anne Tischer - Klinikum Großhadern, LMU München, Chirurgische Klinik, München, Deutschland
  • Andreas Meyer - Klinikum Großhadern, LMU München, Chirurgische Klinik, München, Deutschland
  • Sebastian Nowak - Klinikum Großhadern, LMU München, Klinik für Nuklearmedizin, München, Deutschland
  • Joachim Andrassy - Klinikum Großhadern, LMU München, Chirurgische Klinik, München, Deutschland
  • Markus Guba - Klinikum Großhadern, LMU München, Chirurgische Klinik, München, Deutschland
  • Peter Bartenstein - Klinikum Großhadern, LMU München, Klinik für Nuklearmedizin, München, Deutschland
  • Karl-Walter Jauch - Klinikum Großhadern, LMU München, Chirurgische Klinik, München, Deutschland
  • Marcus Hacker - Klinikum Großhadern, LMU München, Klinik für Nuklearmedizin, München, Deutschland

Deutsche Gesellschaft für Chirurgie. 127. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 20.-23.04.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10dgch194

doi: 10.3205/10dgch194, urn:nbn:de:0183-10dgch1946

Published: May 17, 2010

© 2010 Herrler et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Introduction: Cell therapy represents a promising therapeutic approach in kidney transplantation. This study examines the precise potentials and limits of cell therapy in renal ischemia-reperfusion injury.

Materials and methods: The effects of endothelial progenitor cell therapy following a 45 min warm ischemia were investigated in a murine model of renal ischemia-reperfusion injury alone and in combination with surgical decompression of the renal compartment. Renal function was measured by 99mTc-MAG3 scintigraphy and laser Doppler perfusion. Structural damage was assessed by histological/ immunohistochemical analysis.

Results: Warm ischemia of 45 min was associated with severe tissue damage and led to a significant decrease in tubular excretion rate (46.4%±12.5%, p<0.05) and renal perfusion (67.7%±3.9%, p<0.001). Cell therapy potently enhanced vascular regeneration with increased perfusion (112.5%±3.1%, p<0.001) and excellent tissue vitality, while tubular excretion remained impaired (33.3%±8.8%, p<0.001). Most importantly, combination of cell-based and decompression therapy enabled significant recovery of renal function (103.3%±16.0%, p<0.05) and perfusion (116.7%±2.5%; p<0.001) and preserved the integrity of renal structures.

Conclusion: Progenitor cell therapy alone promotes vascular regeneration and preserves organ integrity following renal ischemia-reperfusion injury, but lacks beneficial effects on renal function. Combining cellular and decompressive therapy results in enhanced functional recovery and may improve the outcome of renal allografts.