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126. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

28.04. - 01.05.2009, München

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99mTc-MAG3 imaging as a novel strategy for therapy evaluation in a murine model of renal ischemia-reperfusion injury

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  • corresponding author T. Herrler - Chirurgische Klinik, Campus Großhadern, LMU München
  • S. Nowak - Klinik für Nuklearmedizin, LMU München
  • S.F. Leicht - Chirurgische Klinik, Campus Großhadern, LMU München
  • A. Tischer - Chirurgische Klinik, Campus Großhadern, LMU München
  • J. Andrassy - Chirurgische Klinik, Campus Großhadern, LMU München
  • P. Bartenstein - Klinik für Nuklearmedizin, LMU München
  • M. Hacker - Klinik für Nuklearmedizin, LMU München
  • C. Heeschen - Chirurgische Klinik, Campus Großhadern, LMU München

Deutsche Gesellschaft für Chirurgie. 126. Kongress der Deutschen Gesellschaft für Chirurgie. München, 28.04.-01.05.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. Doc09dgch10783

doi: 10.3205/09dgch592, urn:nbn:de:0183-09dgch5922

Published: April 23, 2009

© 2009 Herrler et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Introduction: Acute kidney injury is a frequent and life-threatening disease. Efficient therapeutic strategies to counter disease progression are still missing. A major drawback of experimental therapy evaluation is the current lack of reproducible techniques to assess renal function in murine models of acute renal failure. Therefore, we aimed to establish a 99mTc-MAG3 scintigraphy protocol for the determination of renal function as a solid basis for future therapy studies.

Material and methods: The extent of renal dysfunction following different time periods of ischemia was evaluated in a murine model of ischemia-reperfusion injury using scintigraphy, laser Doppler perfusion, and histological analyses.

Results: 99mTc-MAG3 imaging accurately demonstrated functional consequences of renal ischemia even in long-term follow-up. Ischemia of 35 min resulted in mild decrease of renal function with preserved regenerative capability. In contrast, 40 and 45 min of ischemia led to a marked functional impairment with no evidence for regeneration. Kidney perfusion was unchanged after 35 and 40 min of ischemia, but significantly decreased following 45 min ischemia time. Histological analysis conclusively confirmed functional findings.

Conclusion: Here we show in a murine model of ischemia-reperfusion injury that 99mTc-MAG3 imaging represents a reliable endpoint in experimental studies for therapy evaluation in acute kidney injury. Our model may aid to demonstrate the functional relevance of promising treatment modalities for renal regeneration.

Figure 1 [Fig. 1]