Figure 1: Shema and expression of OSCAR in human cell lines
A: Schema of the OSCAR protein; SP: signal peptide, TM: transmembrane domain, N, C: N, C-glycosylation, A: ITAM motif B: Western blotting with different cell fractions of HUVEC. PECAM, lamin A / C and GAPDH serve as positive controls for the different fractions, C: cytosol; M: membrane, N: nucleus, C: Immunofluorescence detection using monoclonal anti-human OSCAR-antibodies in human endothelial cell line EA.hy926 (63-fold magnification).