Figure 2: Regulation of OSCAR expression in EA.hy926 by endothelial growth factors
A: mRNA expression of OSCAR 48 h after incubation of EA.hy926 serum starved medium and stimulation of cells with 10 ng/ml or 1 ng/ml VEGF, TGF-β and bFGF. The analysis was performed by real-time PCR and evaluation by the ΔΔCT method and is shown as x-fold change relative to the negative control. B: Protein expression of OSCAR 48 h after incubation was carried out as in A. The protein expression was determined by densitometric analysis of Western blots and also shown as x-fold change relative to the negative control. * (p<0.05)