gms | German Medical Science


Figure 2: Representation of the organ culture model of the internal thoracic artery (A) and saphenous vein (B). Immunohistochemical staining with polyclonal and monoclonal antibodies to VEGF-R2, VEGF-R1 and NP-1. Cultivation in 10% FCS added endothelial cell medium with and without VEGF (10 ng/ml) compared with the control preparation before starting cultivation (Preculture). Labeling with biotin-conjugated secondary antibody and streptavidin-alkaline phosphatase conjugate. Color development with Fast Red.