Figure 1: Surgical procedure of the new model, in a female BKS.Cg-Dock7m +/+ Leprdb/J mouse.
A: Depilation of the mouse with razor and depilation cream. B: Marking of the cranio-caudal line to place the dorsal skinfold chamber. C: Disinfection and preparation of the puncture for the insulin pump placement. D: Perforation of skin using a 12G trocar. E: Placing the insulin implant into the trocar. F: Subcutaneous application of the insulin implant. G: Suture of the puncture wound. H–I: Mounting the dorsal skinfold chamber. J: Wounding. K: Infection of the wound with the S. aureus suspension. L: Postoperative appearance.