Figure 3: EPO receptor – immunofluorescence microscopy
HL = Hannover solution, EPO = flaps flushed with EPO before placement in Hannover solution, negativ = negative control, positiv-h = positive control on human vessel, positiv-r= positive control on rat vessel.
Human and rat vessels express EPO receptor, but not rat adipose tissue at any timepoint. Rat adipose tissue without visible vessels was analysed. Immunofluorescence microscopy (magnification 40x) was performed using an Axio Vision microscope with Axia Vision software version 4.6.3, Carl Zeiss, Jena, Germany showing the detectability of the EPO receptor at time-points 0, 8 hours, 24 hours, 48 hours and 240 hours after raising the inguinal fat flap of the rat and placement in the bioreactor.