Figure 4a–c: Cytotoxic activity of T cells against cell lines HL-60 (a), KG-1 (b) and K-562 (c)
CIK cells and DCs were generated from various healthy donors as described in “Materials and methods”. Effector cells were cocultured on day 7 with either non-pulsed DCs or with DCs that had been exposed to blast cell lysates or AML cell-derived RNA, respectively. Lysis of AML blasts was measured in an LDH-release assay on day 14 of cell culture. Effector cells not coincubated with DCs were used as control. (EC, effector cells (control); EC/DC, effector cells cocultured with non-pulsed DCs; EC/DC/lysate, effector cells cocultured with DCs pulsed with blast cell lysate; EC/DC/RNA, effector cells cocultured with DCs pulsed with blast cell-derived total RNA). Results represent the mean of at least three separate experiments (SEM <20% of the mean).