Figure 4: Summary of ejection fraction data
Echocardiography was performed and ejection fraction (%) was calculated from the M-mode measurements as described in Methods. Groups: vehicle-treated sham-operated mice (sham vehicle), vehicle-treated mice following 30 min of ischemia and 4 hr of reperfusion (vehicle) and mice undergoing MI/R that were treated with C5 monoclonal antibody (antiC5) 30 min prior to reperfusion (30preR) or with C3a receptor antagonist (C3aRA) 120 min and 30 min prior to R, respectively (120preR or 30preR). All data are mean ± SE of 4–6 animals per group. * p<0.05 compared to sham-operated animals (sham). ** p<0.05 compared to untreated mice undergoing MI/R with 30 min of ischemia and 4 hr of reperfusion.