Figure 6: PMN infiltration into the myocardium following MI/R
6A: Representative sections. Heart sections were stained and PMN infiltration into the myocardium was measured using an infrared imaging system as described in Methods. Representative sections are shown from 4–6 animals per group. Upper row: 1.+2. Ab (primary and secondary antibody). Lower row: control group with 2. Ab only (secondary only antibody). Groups: vehicle-treated sham-operated mice (sham vehicle), vehicle-treated mice that underwent MI/R with 30 min of I and 4 hr of R (vehicle), mice undergoing MI/R and treated with C5 monoclonal antibody (antiC5) 30 min prior to R (30preR) or with C3a receptor antagonist (C3aRA) 120 min and 30 min prior to R respectively (120preR or 30preR). 6B: Quantitatvie analysis of sections by pixel count. Pictures from heart sections stained for PMNs from 4-6 animals per group were quantitatively analyzed by pixel counting using ImageJ software. * p<0.05 compared to sham-operated animals (sham). ** p<0.05 compared to untreated mice undergoing MI/R (MI/R vehicle).