Figure 2: Kinetics of appearance and activity levels of MMP-2, MMP-1 and TIMPs of primary cell cultures of the pharynx grown on polystyrene (control surface) versus multiblock copolymer surface
Scanning densitometry units of the gelatinolytic activity of MMP-2 are shown in A. MMP-1 activity is represented as units per 108 cells in B. The TIMP activity was calculated as percent inhibition of MMPs (C). MMP-2 levels were determined on Days 1, 3, 9 and 12 of cell growth. MMP-1 and TIMP levels were analysed on Days 1, 6 and 12 of cell growth. Statistical analysis was performed to determine differences in MMP-2, MMP-1 and TIMP levels between Day 1 and the subsequent days of cell growth. Statistically significant differences (p≤0.01) are indicated by a star. Data shown are averages from three separate experiments with 9 cell culture each (values are means ± s.d.).