gms | German Medical Science

International Conference on SARS - one year after the (first) outbreak

08. - 11.05.2004, Lübeck

Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome (SARS) coronavirus

Poster

  • corresponding author presenting/speaker Burtram Fielding - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore
  • Yee-Joo Tan - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore
  • Shen Shuo - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore
  • Timothy H.P. Tan - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore
  • Eng-Eong Ooi - Environmental Health Institute, National Environmental Agency, Singapore
  • Seng-Gee Lim - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore; Dept. of Medicine, National University Hospital, Singapore
  • Wanjin Hong - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore
  • Phuay-Yee Goh - Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore

International Conference on SARS - one year after the (first) outbreak. Lübeck, 08.-11.05.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04sarsP6.01

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/sars2004/04sars115.shtml

Veröffentlicht: 26. Mai 2004

© 2004 Fielding et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential coronavirus replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other coronaviruses, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122 amino acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical ER retrieval motif, KRKTE. U122 was expressed in SARS-CoV infected Vero E6 cells, as it could be detected by Western blot and immunoflourescence analyses. U122 is localized to the perinuclear region of both SARS-CoV infected and transfected cells and co-localized with endoplasmic reticulum (ER) and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional.