gms | German Medical Science

International Conference on SARS - one year after the (first) outbreak

08. - 11.05.2004, Lübeck

M and N gene sequence analyses on the newly occurred SARS CoV in Guangdong

Poster

  • corresponding author presenting/speaker Hui-qiong Zhou - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Kui Zheng - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Qiu-xia Chen - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Xin Zhang - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Huang-ying Zheng - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Hui Li - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Ling Fang - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Xin-ge Yan - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Jie Li - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Li-mei Diao - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Pin Huang - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Hao-jie Zhong - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Ji-cheng Huang - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Zhuo-yue Wan - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China
  • Jin-yan Lin - Microbiology Institute, Centers for Disease Control and Prevention of Guangdong Province, P.R.China

International Conference on SARS - one year after the (first) outbreak. Lübeck, 08.-11.05.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04sarsP4.04

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/sars2004/04sars112.shtml

Veröffentlicht: 26. Mai 2004

© 2004 Zhou et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Background: The first case of SARS was reported in Foshan of Guangdong Province on Nov 16, 2002 and caused a outbreak. One year later, a new case of SARS was reemerged in Guangzhou on Dec 16, 2003. The patient showed a positive IgM and IgG to SARS-CoV in serum, and viral sequences were amplified by Light Cycle PCR in throat specimen, but the isolation of virus from clinical specimen was unsuccessful. The M and N genes were thus sequenced to confirm the newly occurred SARS and look for possible origin.

Methodology: RNA was extracted from the samples and used for RT-PCR by specific M and N gene primers, and the PCR products were used for direct sequencing.

Results: In comparison with published Genbank sequences of SARS CoV, GD03T0013 has a difference of 1 base in M and 4 bases in N, the coded amino acid sequence is the same in M and has a difference of 3 amino acids in N, and two of the amino acids changed from hydrophilic threonine to hydrophobic isoleucine. Compared with the early and middle phase SARS-CoV of the 2002-2003 outbreak, the M gene of GD03T0013 has a nucleotide homology of 99.8% and 99.7%. Compared with GD01, SZ3, palm civetand GD69, the N genes of GD03T0013 has a nucleotide homology of 99.7%, 99.7%and 99.6%. amino acid homology of 99.1%, 99.1%and 98.8%.

Conclusion: The origin of SARS CoV of the new patient may come from the palm civet, The disease showed a less severe symptoms and signs, and caused no human to human transmission. So the virus may belong to a different branch compared with the SARS CoV of the last outbreak.