gms | German Medical Science

International Conference on SARS - one year after the (first) outbreak

08. - 11.05.2004, Lübeck

Experience with laboratory diagnosis of SARS in Czech Republic

Poster

  • Martina Havlickova - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Jaroslav Tacner - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Radomira Limberkova - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Jana Schramlova - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Daniela Brynychova - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Marie Otavova - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • corresponding author presenting/speaker Helena Jirincova - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Jan Kyncl - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
  • Bohumir Kriz - Center of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic

International Conference on SARS - one year after the (first) outbreak. Lübeck, 08.-11.05.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04sarsP1.03

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/sars2004/04sars102.shtml

Veröffentlicht: 26. Mai 2004

© 2004 Havlickova et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Objectives: Severe acute respiratory syndrome (SARS) is a viral respiratory illness caused by a coronavirus, called SARS-associated coronavirus. There is summarised the experience obtained to date with testing patients suspected of the SARS coronavirus infection. Nasopharyngeal swabs, nasopharyngeal lavage fluid, urine, stool, acute and convalescent blood samples were collected from the suspected patients.

Methods: The patients were tested as recommended by the WHO guidelines - the samples were analysed for the presence of SARS coronavirus and other possible viral respiratory pathogens. Based on the WHO requirements, the following criteria had to be met: either at least two different types of specimens positive for SARS-CoV or two SARS-CoV positive specimens of the same type if samples were collected at different time during the infection. Samples had to be unambiguously positive in PCR. Positive results of the same specimen tested by two different procedures had been also taken in consideration as an alternative. Isolation of the virus from the respective cell culture confirmed electron-optically and at least a four-fold increase in antibody level in and convalescent serum in comparison with the acute serum samples were unambiguously indicative of SARS etiology.

Results: Altogether 32 quarantined persons suspected of SARS infection were tested. They either showed positivity for type A influenza virus, respiratory syncytial virus, picornaviruses, herpesviruses, adenoviruses, non-SARS coronaviruses. Mycoplasma pneumoniae, Chlamydia pneumoniae and other bacterial infections were tested as negative. Only one of the patients tested was reported by the standard way as a SARS suspected case. Since SARS-CoV infection was not confirmed by following tests, the patient was excluded from the WHO and EU databases. This severe case of respiratory infection with a positive travel history is presented as a case report.

Conclusion: The test system used enabled establishment of preliminary laboratory diagnosis within 24 hours from patient admission and consequently institution of adequate therapy and adoption of anti-epidemic measures. SARS infection was not detected in any of the patients tested. The algorithm used in patient laboratory testing proved as an effective in practice.