Artikel
Outcome prediction of RCC patients with a tumor thrombus of the inferior vena cava: A moleculobiological approach using microRNA expression
Suche in Medline nach
Autoren
-
D. Vergho
- Klinik und Poliklinik für Urologie und Kinderurologie, Julius Maximilians-Universität, Würzburg, Würzburg, Germany
-
C. Kalogirou
- Klinik und Poliklinik für Urologie und Kinderurologie, Julius Maximilians-Universität, Würzburg, Würzburg, Germany
-
S. Kneitz
- Institut für Physiologische Chemie, Würzburg, Germany
-
M. Spahn
- Klinik und Poliklinik für Urologie, Universität Bern, Bern, Switzerland
-
A. Löser
- Klinik und Poliklinik für Urologie und Kinderurologie, Julius Maximilians-Universität, Würzburg, Würzburg, Germany
-
A. Rosenwald
- Institut für Pathologie der Universität Würzburg, Würzburg, Germany
-
H. Riedmiller
- Klinik und Poliklinik für Urologie und Kinderurologie, Julius Maximilians-Universität, Würzburg, Würzburg, Germany
-
B. Kneitz
- Klinik und Poliklinik für Urologie und Kinderurologie, Julius Maximilians-Universität, Würzburg, Würzburg, Germany
| Veröffentlicht: | 23. April 2013 |
|---|
Gliederung
Text
Objective: MicroRNAs (miRs) have emerged as a powerful approach to characterize malignant diseases including renal cell cancer (RCC). We analyzed the expression of nine previously described potential oncomirs in patients with and without tumor thrombus (TT) of inferior vena cava (IVC) to evaluate the potential as diagnostic and prognostic tools in this heterogenous disease regarding reliable outcome prediction of the patient.
Methods: We isolated miRNA from RCC samples with (n=46) or without (n=16) TT of IVC and non-neoplastic renal tissue (n=16). All patients underwent radical surgical treatment between 1997 and 2009. Expression of the known oncomirs miR-21, miR-30a, miR-141, miR-143, miR-200c, miR-210, miR-221, let-7b and let-7c was assessed by qRT-PCR. We compared the normalized miR-expression (using RNU6B) in both tumor groups and the normal tissue and associated expression profiles with clinical parameters and cancer-specific survival using statistical methods (including but not limited to Kaplan-Meier estimation, cox regression models and student´s t-test).
Results: We identified significant dysregulation of eight miRs in RCC when compared to normal renal tissue. Comparing miR-expression in RCC with or without TT of IVC we detected significant differences in the expression of miR-200c, miR-141 and let-7b. Additionally we found that a strong down-regulation of miR-200c was significantly associated with clinical progression, metastasis and cancer-specific survival in patients with TT of IVC.
Conclusions: Using the expression profiles of let-7b and miR-141 we were able to differentiate between tumors with or without TT of IVC. Furthermore, strong down-regulation of miR-200c was significantly associated with cancer-specific death and metastasis in RCC patients with TT of IVC. These results indicate that analyzing miR-expression profiles might be a diagnosic as well as a prognostic tool in patients with and without TT of IVC. We are planning to verify the prognostic validity of miR-200c in an independent study cohort.
