gms | German Medical Science

29. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Hochdruckliga e. V. DHL ® - Deutsche Hypertonie Gesellschaft Deutsches Kompetenzzentrum Bluthochdruck

23. bis 25.11.2005, Berlin

LDL-cholesterol is a Major Determinant of Vascular Oxidative Stress and Endothelial Dysfunction in Patients with Coronary Artery Disease

LDL-Cholesterin bestimmt vaskulären oxidativen Stress und Endothelfunktion in Patienten mit koronarer Herzerkrankung

Meeting Abstract

  • C. Delles - Univesity of Glasgow, Estern Infirmary, Glasgow
  • S. Al-Benna - Univesity of Glasgow, Estern Infirmary, Glasgow
  • L.U. Zimmerli - Univesity of Glasgow, Estern Infirmary, Glasgow
  • J.D. McCllure - Univesity of Glasgow, Estern Infirmary, Glasgow
  • C.A. Hamilton - Univesity of Glasgow, Estern Infirmary, Glasgow
  • A.F. Dominiczak - Univesity of Glasgow, Estern Infirmary, Glasgow
  • A.J. Kirk - Western Infirmary, Glasgow
  • P.N. Rogers - Gartnavel General Hospital, Glasgow

Hypertonie 2005. 29. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Berlin, 23.-25.11.2005. Düsseldorf, Köln: German Medical Science; 2006. Doc05hochP89

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2005/05hoch089.shtml

Veröffentlicht: 8. August 2006

© 2006 Delles et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Background. There is evidence for a relationship between endothelial dysfunction and cardiovascular disease, but a causative role for oxidative stress remains to be determined.

Methods. We studied 188 patients with severe coronary artery disease (CAD), of whom 51 were age and sex matched with 51 healthy controls undergoing varicose vein surgery. Relaxation of saphenous vein to calcium ionophore, apocynin and allopurinol was studied together with the markers of oxidative stress, total antioxidant capacity (TAC) and reduced/oxidised glutathione ratio. Vascular superoxide levels were measured using lucigenin chemiluminescence and hydroethidine. Relationships between risk factors, endothelial function and oxidative stress were analysed using multiple linear regression.

Results. Relaxation to calcium ionophore was decreased in CAD compared to control patients (maximum relaxation 26±2 vs 60±1%; P<0.001; Fig. 1a [Fig. 1]). Total superoxide production was increased (0.89±0.09 vs 0.56±0.06 nmol/mg/min, P=0.008; Fig. 1b [Fig. 1]). NAD(P)H oxidase, xanthine oxidase and eNOS were sources of excess superoxide in CAD patients. LDL-cholesterol was a significant predictor of relaxation to calcium ionophore (P<0.001) and oxidative stress markers (P<0.001; Fig. 1c [Fig. 1]) in CAD patients. Diastolic blood pressure was also a significant determinant of oxidative stress. Allopurinol had a greater effect on maximum vasorelaxation in diabetic than non-diabetic patients (35±3 vs 21±1%, P<0.001).

Conclusions. Endothelial dysfunction is associated with increased levels of superoxide and biomarkers of oxidative stress in CAD patients. LDL-cholesterol is a major determinant of endothelial dysfunction and oxidative stress in these patients. These results give mechanistic rationale for intensive LDL-cholesterol lowering therapy as suggested by recent clinical trials