gms | German Medical Science

28. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

24. bis 27.11.2004, Hannover

Depletion of Adiponectin in Pericardial-Fat Tissue: A Potential Role for Cardiac MMP-Regulation

Depletion von Adiponektin im Perikardialen Fettgewebe: Potentielle Funktionen für die Kardiale MMP-Regulation

Meeting Abstract (Hypertonie 2004)

  • R. Clasen - Center for Cardiovascular Research, (Berlin, D)
  • C. Sprang - Center for Cardiovascular Research, (Berlin, D)
  • M. Clemenz - Center for Cardiovascular Research, (Berlin, D)
  • M. Krikov - Center for Cardiovascular Research, (Berlin, D)
  • C. Thöne-Reineke - Center for Cardiovascular Research, (Berlin, D)
  • T. Unger - Center for Cardiovascular Research, (Berlin, D)
  • U. Kintscher - Center for Cardiovascular Research, (Berlin, D)

Hypertonie 2004. 28. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Hannover, 24.-27.11.2004. Düsseldorf, Köln: German Medical Science; 2005. Doc04hochP28

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2004/04hoch028.shtml

Veröffentlicht: 10. August 2005

© 2005 Clasen et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Visceral fat-accummulation has been associated with an increased cardiovascular risk including the development of heart failure. However, functional differences between distinct visceral fat depots (e.g. pericardial fat, epididymal fat) are unknown. The adipose-specific protein adiponectin has been demonstrated to exert direct effects on cardiovascular cells. In the present study we investigated the expression of adiponectin protein in pericardial vs. epididymal adipose tissue in obese Zucker rats. In rat cardiac fibroblast we studied the regulation of matrix metalloproteinase (MMP)-9 by adiponectin.

Male Obese zucker rats (n=6) were killed at 11 weeks of age (mean body weight: 415+/-9.2g, mean adiponecin serum level: 6.3+/-0.6µg/ml), and epididymal and pericardial fat depots were removed for protein isolation. Adiponectin protein expression was reduced by 52.2+/-12.4% (p<0.01) in the pericardial fat depots compared to epididymal fat depots, as assessed by western immunoblotting. To identify a function of locally produced adiponectin, we investigated the regulation of MMP-9 expression in primary rat cardiac fibroblasts. Cardiac fibroblasts were stimulated with angiotensin II (AngII 100nmol/L) +/- adiponectin (5µg/ml) and MMP-9 protein expression (88 and 92kDa) was measured after 24h. AngII stimulated MMP-9 expression by 2.2+/-0.2-fold (p<0.05 vs. vehicle-treated cells). AngII-induced MMP-9 expression was potently blocked by 51+/-15.5% (p<0.05 vs. AngII alone) in the presence of adiponectin.

The present study identifies a distinct endocrine function of pericardial fat tissue compared to other visceral fat depots. Depletion of adiponectin expression in pericardial fat tissue may play an important role in the regulation of cardiac MMP expression, which may affect cardiac remodelling during obesity.