gms | German Medical Science

27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Liga zur Bekämpfung des hohen Blutdrucks – Deutsche Hypertonie Gesellschaft e. V.

26. bis 29.11.2003, Bonn

AT1/AT2 receptor subtype expression in peripheral blood monocytes from healthy normals and hypertensive patients

Expression von AT1/AT2 - Rezeptorsubtypen durch periphere Monozyten von Normalpersonen und Patienten mit arterieller Hypertonie

Meeting Abstract (Hypertonie 2003)

Suche in Medline nach

  • presenting/speaker A. Hansen - Universitätsklinikum Charité (Berlin, D)
  • K. Reiter - Universitätsklinikum Charité (Berlin, D)
  • M. Lehmann - Universitätsklinikum Charité (Berlin, D)
  • J. Scholze - Universitätsklinikum Charité (Berlin, D)

Hypertonie 2003. 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Bonn, 26.-29.11.2003. Düsseldorf, Köln: German Medical Science; 2004. Doc03hochP85

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2003/03hoch185.shtml

Veröffentlicht: 11. November 2004

© 2004 Hansen et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Activated monocytes contribute to vascular damage in hypertension/atherosclerosis. Angiotensin II (ATII) has been shown to be a potent activator of blood monocytes, particularly in hypertensives. However, ATII receptor expression in blood monocytes has been rarely investigated. Thus, the current study analyzed the expression of ATII receptor subtypes AT1R and AT2R in peripheral blood monocytes from healthy normals and hypertensive patients, both at protein and mRNA level. Peripheral mononuclear cells were obtained from 10 normals and 10 untreated patients with essential hypertension (stage I-II, WHO) by ficoll-paque gradient centrifugation and subsequently analyzed by fluorescence-activated cell sorting. Briefly, CD14+ monocytes were gated and analyzed for percentages of AT1R+ and AT2R+ cells, respectively. About half of the blood monocytes from both normals (54.4±22.6%, mean±SD) and hypertensives (48.8±20.2%) stained positive for surface AT1R, whereas significantly (p<0.05) lower percentages stained positive for surface AT2R in both groups, i.e. normals (17.0±10.9%) and patients (28.1±18.5). However, no significant differences in either AT1R or AT2R surface expression were found between groups. On mRNA level, both AT1R and AT2R specific transcripts were detected in blood monocytes from normals and patients. Of note, short-time in-vitro stimulation with ATII resulted in down-regulation of AT1R mRNA expression in blood monocytes from hypertensives but not in those from normal subjects (p=0.028), as was detected by semi-quantitative real-time PCR. Whether therapy with ACE inhibitors or AT1 receptor blockers in hypertensive patients results in changes of AT1R/AT2R expression has to be examined in further studies.