gms | German Medical Science

27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Liga zur Bekämpfung des hohen Blutdrucks – Deutsche Hypertonie Gesellschaft e. V.

26. bis 29.11.2003, Bonn

Validation of a new Laser Doppler Scanner device for the assessment of in vivo endothelial function in human microcirculation

Validierung eines neuen Laser Doppler Scanners zur Messung der Endothelfunktion in der menschlichen Mikrozirkulation in vivo

Meeting Abstract (Hypertonie 2003)

  • presenting/speaker A.M. Opazo Saez - Universitätsklinik Essen (Essen, D)
  • F. Mosel - Universitätsklinik Essen (Essen, D)
  • U. Rushentsova - Universitätsklinik Essen (Essen, D)
  • R.F. Schäfers - Universitätsklinik Essen (Essen, D)
  • T. Philipp - Universitätsklinik Essen (Essen, D)
  • R.R. Wenzel - Universitätsklinik Essen (Essen, D)

Hypertonie 2003. 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Bonn, 26.-29.11.2003. Düsseldorf, Köln: German Medical Science; 2004. Doc03hochP47

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2003/03hoch147.shtml

Veröffentlicht: 11. November 2004

© 2004 Opazo Saez et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Background

Laser Doppler flowmetry is used in various fields of medicine for the evaluation of blood flow in the very small blood vessels of the microvasculature. The purpose of this study was to evaluate the accuracy and reproducibility of a new laser Doppler imager (LDI) as a tool for the assessment of endothelial function in human skin microcirculation. In contrast to other devices, the LDI measures a large area instead of a single point measurement allowing the assessment of in vivo dose-response-curves simultaneously.

Methods

We used a Laser Doppler imager (moorLDI V3.0) to continuously monitor the change in blood flow during intradermal administration of acetylcholine (ACh 10-7, 10-8, 10-9 mol) and endothelin-1 (ET-1 10-14, 10-16, 10-18 mol) in 10 healthy male subjects. Subjects were examined on three different days and injections of either drug were randomly placed on different sites of the forarm. Double injections of NaCl and either ACh or ET-1 were performed. Laser Doppler images were taken before and after injection at 2 min intervals for 30 min. Data were analyzed offline after completion of each experiment using Moor Software V.3.01. The investigator was blinded for the drug under investigation.

Results

Interday variability as assessed by the coefficient of variation on the same site, in the same individual was 20% for ACh, and 15% for ET-1. Dynamic and steady state responses were consistent with known pharmacology. Inter-observer variability was 9%.

Conclusion

Endothelium-dependent responses of skin blood flow evaluated with laser Doppler imaging are reproducible from day to day in young healthy male subjects. The low interobserver variability indicates that the new LDI device is not user dependent. These results suggest that this technique may be a promising tool for in vivo pharmacology.

Results

Interday variability as assessed by the coefficient of variation on the same site, in the same individual was 20% for ACh, and 15% for ET-1. Dynamic and steady state responses were consistent with known pharmacology. Inter-observer variability was 9%.