gms | German Medical Science

27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Liga zur Bekämpfung des hohen Blutdrucks – Deutsche Hypertonie Gesellschaft e. V.

26. bis 29.11.2003, Bonn

Effect of gender and estradiol on the expression Ang II type-2 (AT2) receptors in the rat kidney

Einfluss von Geschlecht und Estradiol auf die Expression von Ang II Typ-2 (AT2) Rezeptoren in der Ratten-Niere

Meeting Abstract (Hypertonie 2003)

Suche in Medline nach

  • presenting/speaker L.A. Campos - Max-Delbrück-Centrum für Molekulare Medizin (Berlin, D)
  • R. Iliescu - Max-Delbrück-Centrum für Molekulare Medizin (Berlin, D)
  • O. Baltatu - Max-Delbrück-Centrum für Molekulare Medizin (Berlin, D)
  • M. Bader - Max-Delbrück-Centrum für Molekulare Medizin (Berlin, D)

Hypertonie 2003. 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Bonn, 26.-29.11.2003. Düsseldorf, Köln: German Medical Science; 2004. Doc03hochV17

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2003/03hoch017.shtml

Veröffentlicht: 11. November 2004

© 2004 Campos et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Background

Sex differences in renal hemodynamics and in the occurrence and progression of renal disease are well recognized. This gender dependency is also apparent in the renal response to Ang II. However, although AT1 receptors have been shown to be regulated by estrogens in various tissues, the effect of gender and estrogens on renal Ang II receptor expression has not been clarified. Therefore, we analyzed possible gender-related differences in the gene expression of renal angiotensin (Ang II) receptors and of Ang II converting enzyme (ACE) and their regulation by estrogens in female rats.

Methods

The mRNA levels of AT1A, AT1B and AT2 receptors and ACE were measured in the kidneys of male and female rats by quantitative real-time RT-PCR. In addition, female rats were either sham-operated or ovariectomized and subsequently treated with placebo or 17beta-estradiol for 21 days and the effect of estradiol on the renal mRNA expression of Ang II receptor subtypes and ACE were analyzed.

Results

In female rats, the mRNA levels of renal AT2 receptors were significantly elevated while the mRNA levels of ACE were significantly reduced compared to male rats. No differences were observed in the mRNA levels of renal AT1A and AT1B receptors between male and female rats. Ovariectomy significantly decreased the mRNA levels of renal AT2 receptors compared to sham-operated rats. In contrast, renal ACE mRNA was increased after ovariectomy. Treatment with 17beta-estradiol reversed the effects of ovariectomy on the AT2 receptor and ACE mRNA.

Conclusions

Besides the gender differences in renal ACE, renal AT2 receptors are increased in female rats and endogenous estradiol appears responsible for these differences. The known gender differences of the renal response to Ang II may partially depend on the differential expression of renal AT2 receptors.