gms | German Medical Science

83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

16.05. - 20.05.2012, Mainz

Feasibility study of postauricular hypodermic injection in curing inner ear disorders by pharmacokinetic comparison using MRI with gadolinium as a tracer

Meeting Abstract

Suche in Medline nach

  • corresponding author Lisheng Yu - People’s Hospital, Peking University, Beijing, P.R. China

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hnod577

doi: 10.3205/12hnod577, urn:nbn:de:0183-12hnod5770

Veröffentlicht: 4. April 2012

© 2012 Yu.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

For patients with inner ear ailments, treatments with systemic administration or intratympanic administration are considered applicable. However, systemic route can't be used because of difficulties with blood-labyrinth barrier and systemic side effects while the intratympanic approach is not reliable sometimes on account of troubles with the variation of the round window membrane (RWM). To investigate its feasibility of delivering therapeutics to the inner ear, we compared the postauricular (p.a.) and intravenous (i.v.) pharmacokinetics in the inner ear fluid with magnetic resonance imaging (MRI) by using a T1 contrast agent Gadopentetate Dimeglumine (Gd-DTPA) as in vivo tracer. Normative data were obtained from male albino guinea pigs with weight ranging from 300 to 400 grams (N = 12), which were divided randomly into two groups and each was given a p.a. or i.v. bolus of Gd-DTPA. A Bruker Biospec Avance 70/30 experimental MRI system with a magnetic field strength of 7.0 Tesla was used to obtain the signal intensity of Gd-enhanced image of the scala tympani (ST) as a biomarker for concentration changes in the perilymph for 48 h. It was Gd-enhanced signal other than perilymph concentration that being calculated for pharmacokinetic parameters. Results revealed longer time to peak signal intensity (Tmax), elimination half-life (T1/2) and mean residence time (MRT), and higher levels of area under the signal time curve from pre-injection to the last time point (AUClast) of postauricularly treated guinea pigs than intravenously treated guinea pigs (p<0.05). The present study demonstrates the use of the novel topical administration—p.a. injection—possibly as a potential and efficient drug delivery route to cure inner ear disorders.