gms | German Medical Science

82. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

01.06. - 05.06.2011, Freiburg

Pluripotency and differentiation potential of vestibular stem cells

Meeting Abstract

  • corresponding author Roxana Vintila - Univ. Victor Babes, Dept. ENT, Timisoara, Rumänien
  • Oana Gavriliuc - Univ. Victor Babes, Dept. ENT, Timisoara, Rumänien
  • Gheorghe Iovanescu - Univ. Victor Babes, Dept. ENT, Timisoara, Rumänien
  • Stan Cotulbea - Univ. Victor Babes, Dept. ENT, Timisoara, Rumänien
  • Calin Tatu - Univ. Victor Babes, Dept. ENT, Timisoara, Rumänien

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 82. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Freiburg i. Br., 01.-05.06.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11hnod502

doi: 10.3205/11hnod502, urn:nbn:de:0183-11hnod5025

Veröffentlicht: 19. April 2011

© 2011 Vintila et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: The purpose of the ongoing research is to improve our current skills and knowledge in stem cell isolation, cultivation and differentiation from the utricular and saccular epithelia of young mice.

Matherials and methods: We harvested utricles and sacculi from 7 days old NMRI mice. Utricles were trypsinized in order to isolate single cells. Obtained cells were cultivated at 37ºC and 5% CO2 in DMEM with F12 Nutrient mixture, B27, N2 supplement, IGF-1 and EGF. We mechanically dissociated primary spheres and cultivated them in the culture media mentioned above. Secondary spheres were placed on fibronectin coated tissue culture slide chambers in the absence of IGF-1 and EGF in order to observe their subsequent proliferation and differentiation. Cells were characterized by immunofluorescence for myosinVIIA (hair cell marker), nestin (intermediate filament VI marker) and beta III tubulin.

Results: We proved that vestibular epithelia contains pluripotent stem cells which were able to form cell clusters called spheres. Sphere-derived cells’ pluripotency was demonstrated by the expression of nanog, oct 4 and nestin markers (cell progenitors). Single spheres harvested and cultivated on fibronectin gave rise through differentiation to different cell types including neuron like-cells which were positive for myosin VIIA, nestin and beta III tubulin.

Conclusions: Utricular epithelia of seven days old mice contains sufficient pluripotent stem cells which, under special conditions, can generate spheres.

Cells obtained from utricular epithelia are pluripotent because they manifest markers fro nanog, oct 4 as well as markers for nestin gene, characteristic for cell progenitors.