gms | German Medical Science

81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

12.05. - 16.05.2010, Wiesbaden

Characterization of HPV-positive head and neck squamous cell carcinoma cell lines

Meeting Abstract

  • corresponding author Nadine Olthof - Maastricht University Medical Centre, Maastricht, The Netherlands
  • Thomas Hoffmann - University of Duisburg-Essen, Germany
  • Jens Klussmann - Justus-Liebig-University Gießen, Germany
  • Bernd Kremer - Maastricht University Medical Centre, Maastricht, The Netherlands
  • Mieke Henfling - Maastricht University Medical Centre, Maastricht, The Netherlands
  • Frans Ramaekers - Maastricht University Medical Centre, Maastricht, The Netherlands
  • Ernst-Jan Speel - Maastricht University Medical Centre, Maastricht, The Netherlands

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Wiesbaden, 12.-16.05.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10hnod196

DOI: 10.3205/10hnod196, URN: urn:nbn:de:0183-10hnod1969

Veröffentlicht: 22. April 2010

© 2010 Olthof et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Antiviral therapy has a possible beneficial therapeutic effect in patients with human papillomavirus (HPV)-positive head and neck squamous cell carcinomas (HNSCC). Because the cellular features responsible for this effect are not sufficiently understood, it is essential to investigate HPV-positive and -negative tumor cell lines. To what extent the genomic and phenotypic features of these cell lines correspond to those of primary tumors is unknown and has been investigated in this study.

Methods: Three HPV-16-positive and 4 HPV-negative HNSCC cell lines were analyzed, and the obtained results were compared with data previously gathered on primary tumor samples. HPV type and physical status were determined using fluorescence in situ hybridisation (FISH), PCR and amplification of papillomavirus oncogenic transcripts (APOT) analysis. Chromosome 1 and 7 copy numbers were determined using FISH. Expression of proteins in the pRb- and p53-pathways and cytokeratines were determined using immunocytochemistry.

Results: Our experiments revealed that 1) all HPV-positive cell lines have punctate FISH patterns indicating integrated oncogenic HPV, and that the integration status was confirmed in 2 cell lines by APOT, 2) the HPV-positive cell lines exhibit aneusomy for chromosomes 1 and 7, 3) HPV influences expression of proteins in the pRb- and p53-pathways in the cell lines in a similar way as in primary tumors and 4) cytokeratine immunostainings indicate a squamous epithelial origin of the cell lines.

Conclusions: The investigated HPV-positive HNSCC cell lines are suitable for research on the therapeutical effects of antiviral therapy.