gms | German Medical Science

79. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

30.04. - 04.05.2008, Bonn

In vivo real time imaging of head neck squamous cancer-cell trafficking in mice

Meeting Abstract

  • corresponding author Johannes Wessels - Universitätsmedizin Göttingen, Göttingen
  • Ann-Christin Busse - Diagn. Radiologie, Universitätsmedizin, Göttingen
  • Wiebke Laffers - Universitätsklinik für Hals-Nasen-Ohrenheilkunde, Bonn
  • Jens Mahrt - Nephrologie/Rheumatologie, Universitätsmedizin, Göttingen
  • Phug Nuygen - Nephrologie/Rheumatologie, Universitätsmedizin, Göttingen
  • Andreas Gerstner - Universitätsklinik für Hals-Nasen-Ohrenheilkunde, Göttingen
  • Gerhard Anton Müller - Nephrologie/Rheumatologie, Universitätsmedizin, Göttingen

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 79. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Bonn, 30.04.-04.05.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. Doc08hnod152

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hnod2008/08hnod152.shtml

Veröffentlicht: 22. April 2008

© 2008 Wessels et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Progression of head neck squamous cell cancer (HNSC) remains regional for long time and metastases develop only in late stages of the disease. Real time imaging of local invasion and the lymphatic dissemination have critical importance for a better understanding of the underlying processes.

To study cancer cell dynamics in vivo the human HNSC cell line FADU was transfected with different mammalian expression vectors encoding different fluorescent proteins. FADU cells were transfected using different vectors. All vectors contain immediate early promoter of CMV for protein expression and SV40 origin for replication in mammalian cells. G418 resistance allows selecting stable transformants. Transfected cells were xenotransplanted into nude mice. Visualization of tumor growth was performed using flat panel volumetric computed tomography (fpVCT). In vivo cell trafficking of HNSC cells was observed by the new imaging system OV100 (Olympus).

All mice developed HNSC- tumours within 14 days after transplantation. The high spatial resolution of the fp-VCT system can be used to accurately determine solid tumor volume, thus allowing for earlier assessment of the therapeutic response. TurboGFP and KATUSHKA showed brighter fluorescence due to extra fast protein expression allowing earlier signal detection compared to eGFP and DsRED. Single labeled HNSC cells could be observed in real time using an abdominal skin flap in nude mice. We established a model to study HNSC cells in vivo and describe tools for early online recognition of HNSC cancer. In vivo cell trafficking gave evidence for the behaviour of tumor cells. Combining molecular and imaging technologies will now enable further understanding of the molecular mechanisms and provide visible targets for drug development.