gms | German Medical Science

79. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

30.04. - 04.05.2008, Bonn

Metallic Nanoparticles and Cochlear Implant Electrode Materials

Meeting Abstract

  • Priya Gomes - HNO-MHH, Hannover
  • Michael Braun - HNO-MHH, Hannover
  • corresponding author Gerrit Paasche - HNO-MHH, Hannover
  • Anne Hahn - Laserzentrum Hannover, Hannover
  • Thomas Lenarz - HNO-MHH, Hannover
  • Stephan Barcikowski - Laserzentrum Hannover, Hannover
  • Timo Stöver - HNO-MHH, Hannover

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 79. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Bonn, 30.04.-04.05.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. Doc08hnod052

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hnod2008/08hnod052.shtml

Veröffentlicht: 22. April 2008

© 2008 Gomes et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

The increase of the impedance at the intracochlear electrode contacts after implantation is typically explained by the formation of fibrous tissue around the electrode array. Silver ions are known to have anti-proliferative and biostatic effects. In the current project these effects shall be transferred to cochlear implants by incorporation of metallic nanoparticles into the silicone of the electrode array in order to reduce the formation of fibrous tissue around the electrode.

Metallic nanoparticles are generated by using short pulses of a fs-laser. The particles are then incorporated into the polymer component of the electrode array during the mixing process of the silicone components. To determine biologically active concentrations of ions released from these nanoparticles, concentrations of metal ions between 0.1 µM and 1 mM were tested on cultured fibroblasts (NIH-3T3), PC-12 cells and freshly isolated spiral ganglion cells (SGC; rats p3-5). Viability of cells was evaluated after 24 and 72 hours by using neutral red assay (NIH-3T3 and PC-12). SGC were fixed, stained (neurofilament) and counted. Furthermore, very first silicone samples containing silver nanoparticles were also investigated in cell culture experiments.

Results showed reduced viability of PC-12 cells as well as fibroblasts at high concentrations of silver nitrate (30 µM) and copper sulphate (300 µM). However, silver nanoparticles immersed in silicone at the concentrations used in our preliminary experiments did not have any effect on PC-12 cells.

Based on our results, size and concentration of nanoparticles in the silicone have to be adjusted to the required concentration in the surrounding medium to reduce the formation of fibrous tissue.

Unterstützt durch: SFB/Transregio 37 TP C4 and FWO Belgium