gms | German Medical Science

81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

12.05. - 16.05.2010, Wiesbaden

Ultrasound-based growth analysis of cultured chondrocyte aggregates

Meeting Abstract

  • presenting/speaker Stephanie Akinyemi - Klinik und Poliklinik für HNO, Universitätsklinikum des Saarlandes, Homburg, Germany
  • Christina Körbel - Institut für Klinisch-Experimentelle Chirurgie, Universitätsklinikum des Saarlandes, Homburg, Germany
  • author Vivienne Willnecker - Klinik und Poliklinik für HNO, Universitätsklinikum des Saarlandes, Homburg, Germany
  • Matthias Laschke - Institut für Klinisch-Experimentelle Chirurgie, Universitätsklinikum des Saarlandes, Homburg, Germany
  • Monika Hoffmann - Klinik und Poliklinik für HNO, Universitätsklinikum des Saarlandes, Homburg, Germany
  • Michael Menger - Institut für Klinisch-Experimentelle Chirurgie, Universitätsklinikum des Saarlandes, Homburg, Germany
  • Bernhard Schick - Klinik und Poliklinik für HNO, Universitätsklinikum des Saarlandes, Homburg, Germany
  • corresponding author Andreas Naumann - Klinik und Poliklinik für HNO, Universitätsklinikum des Saarlandes, Homburg, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 81st Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Wiesbaden, 12.-16.05.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10hno112

DOI: 10.3205/10hno112, URN: urn:nbn:de:0183-10hno1126

Veröffentlicht: 6. Juli 2010

© 2010 Akinyemi et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: The subject of the present study is the in vivo growth behavior of in vitro cultured chondrocyte aggregates. The aim of this study was a repetitive, non-invasive volume determination of engineered cartilage tissue based on human chondrocytes in a mouse model by using 30 MHz ultrasound technology.

Methods: Cartilage cells were isolated from human nasal septum cartilage (n=10; m=6/w=4) and expanded for 3 weeks in a monolayer culture. Then the isolated chondrocytes were cultured for further 3 weeks in an in vitro aggregate culture system before 48 aggregates were implanted subcutaneously in an athymic nude mouse model for 4 and 8 weeks respectively. The aggregate volume was analyzed over time by using the high-resolution, 3-D ultrasound imaging system Vevo770TM (Visual Sonics, Toronto, ON, Canada).

Results: First results of collected data showed both, donor-site specific differences in aggregate volumes during in vivo cultivation as well as volume variations within a collective over the 8-week period of study.

Conclusion: The documented volume variations of specific cartilage specimen in 3D aggregates show that, based on a constant number of cells, there are variations in regard to in vivo behavior of individual transplants of in vitro cultured cartilage.

Supported by: HomFor