gms | German Medical Science

81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

12.05. - 16.05.2010, Wiesbaden

The relevance of polymorphisms of toll-like receptors (TLR) in chronic rhinosinusitis

Meeting Abstract

  • corresponding author presenting/speaker Martin Wagenmann - HNO-Klinik, Universität Düsseldorf, Germany
  • Adam Chaker - HNO-Klinik, Universität Düsseldorf, Germany
  • Simone Hindersin - HNO-Klinik, Universität Düsseldorf, Germany
  • Jörg Schipper - HNO-Klinik, Universität Düsseldorf, Germany
  • Kathrin Scheckenbach - HNO-Klinik, Universität Düsseldorf, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 81st Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Wiesbaden, 12.-16.05.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10hno102

DOI: 10.3205/10hno102, URN: urn:nbn:de:0183-10hno1024

Veröffentlicht: 6. Juli 2010

© 2010 Wagenmann et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Immune responses of the innate and adaptive immune systems can be influenced by polymorphic variants of TLRs. Significant associations between polymorphisms of TLR2, TLR4, TLR5, and TLR9 and the development of asthma and atopic diseases have been described. The aim of our study was to analyze potential connections between single nucleotide polymorphisms (SNPs) in chronic rhinosinusitis with (CRS+NP) and without nasal polyps (CRS-NP).

Methods: Samples from 240 patients (CRS+NP (n=98), CRS-NP (n=85), controls (n=57)) were analyzed. DNA was obtained from tissue or blood samples. After amplification, direct sequencing of regions with TLR-polymorphisms in sense and anti-sense-direction were performed. Results were confirmed by repetition of the sequencing. We investigated 25 SNPs of TLR2, 4, 5, and 9 that were statistically analyzed using chi-square tests.

Results: The T-variant of SNP rs 4986791 (Thr399Ile) of TLR4 was significantly more often in patients with CRS+NP than in patients with CRS-NP (p=0.0226) and significantly more frequent than in the published distribution of the caucasian population (HapMap) (p=0.0169). Comparisons between CRS-NP and controls as well as CRS-NP and the published normal distribution showed no significant differences. For all other investigated SNPs of TLR4 and the other SNPs of TLR2, TLR5, and TLR9 no significant differences between groups and the published normal distribution no significant differences could be observed.

Conclusion: The T-variant of the SNP rs 4986791 (Thr399Ile) of TLR4 seems to be associated with a predisposition to develop chronic rhinosinusitis with nasal polyps.