gms | German Medical Science

81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

12.05. - 16.05.2010, Wiesbaden

RNase 7 – part of the innate immune system of the nasal mucosa

Meeting Abstract

  • corresponding author Alexander Bremer - Department of Otorhinolaryngology, Head- and Neck surgery, Christian-Albrechts- University Kiel, Germany
  • author Janet Wohlers - Department of Otorhinolaryngology, Head- and Neck surgery, Christian-Albrechts- University Kiel, Germany
  • author Rainer Podschun - Institute of Infection Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • author Peter Lamprecht - University of Lübeck, Department of Rheumatology, Vasculitis Center UKSH and Clinical Center Bad Bramstedt, Lübeck, Germany
  • author Petra Ambrosch - Department of Otorhinolaryngology, Head- and Neck surgery, Christian-Albrechts- University Kiel, Germany
  • author Jürgen Harder - Department of Dermatology, Allergology and Venerology, University Hospital of Schleswig-Holstein, Campus Kiel, Germany
  • author Regina Gläser - Department of Dermatology, Allergology and Venerology, University Hospital of Schleswig-Holstein, Campus Kiel, Germany
  • author Martin Laudien - Department of Otorhinolaryngology, Head- and Neck surgery, Christian-Albrechts- University Kiel, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 81st Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Wiesbaden, 12.-16.05.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10hno089

DOI: 10.3205/10hno089, URN: urn:nbn:de:0183-10hno0898

Veröffentlicht: 6. Juli 2010

© 2010 Bremer et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Ribonuclease (RNase) 7 is a cationic peptide with broad antimicrobial activity including Staphylococcus (S.) aureus and is detectable e.g. in epithelia of the skin and intestine. Hitherto nasal secretion has not been investigated in respect to RNase 7 concentration although inhaled microorganisms have first contact with the immune system in the nasal cavity.

Patients and methods: The RNase 7 concentration in nasal secretions of patients with Wegeners Granulomatosis (WG, n=12), Polyposis nasi (PP, n=11) and healthy controls (n=12) as well as RNase 7 concentration in the serum of 14 WG, 12 PP and 13 healthy controls was analyzed by ELISA. Primary nasal epithelial cell cultures (NEC) of healthy volunteers were stimulated with S. aureus and the concentration of RNase 7 in the supernatants have been determined.

Results: RNase 7 was detectable in nasal secretions of all three groups. The differences were not significant. In serum RNase 7 was verified in 14 of 35 probes and uniformly distributed among the groups. After stimulation of NEC with increasing concentrations of S. aureus the RNase 7 concentration in the supernatant decreased.

Discussion: RNase 7 is part of the innate immune system of the nasal mucosal barrier and rarely detectable systemically. Referring to our data no defect of the nasal barrier was detectable for WG and PP. An inhibiting effect for increasing concentrations of bacteria seems functionally possible. Further investigation particularly regarding possible interactions between microorganisms and epithelial cells (bacterial peptidases, inhibiting components in nasal secretion, interaction of antimicrobial peptides) will follow.


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