gms | German Medical Science

80. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

20.05. - 24.05.2009, Rostock

Marking of lasermicrosurgical resection specimens

Meeting Abstract

Suche in Medline nach

  • corresponding author Martin C. Jäckel - ENT Department, Schwerin, Germany
  • Agnes Bankfalvi - Institute of Pathology, Essen, Germany
  • Raoul Hinze - Institute of Pathology, Schwerin, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 80th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Rostock, 20.-24.05.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. Doc09hno023

doi: 10.3205/09hno023, urn:nbn:de:0183-09hno0234

Veröffentlicht: 22. Juli 2009

© 2009 Jäckel et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen ( Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.



Introduction: Lasermicrosurgery of tumors of the upper aerodigestive tract is often done by the so-called piecemeal technique, i.e. tumors are removed in several pieces. In this situation the basal margins of resection specimens have to be marked by the surgeon to facilitate orientation of the pathologist. Steiner and Ambrosch originally recommended marking by a pen, which has the disadvantage that it has to be renewed by the pathologist before further work-up of specimens. This may result in a wrong assessment of resection margins.

Method: On the unfixed specimen the surgeon marks the deep resection margin with the histological dye WAK-HM-G-1 (WAK-Chemie Medical GmbH, Steinbach, Germany). The dye is applied without dilution by a small cotton bud under microscopic vision. After swabbing excesses of the dye, the specimens are fixed in formalin, sectioned perpendicular to the marked margin and stained with hematoxylin and eosin.

Result: The dye-marking remains stable during histological processing and does not run to other margins of the specimen. In the histological section the basal margin can be identified by a thin green strip which contrasts well with the HE stain (Figure 1 [Fig. 1]).

Conclusion: The procedure allows a reliable and distinct marking of lasermicrosurgical resection specimens. Its key benefit is that the surgeon alone determines definitely which margins are investigated by the pathologist.