gms | German Medical Science

78. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

16.05. - 20.05.2007, München

Expression of gelatinases and their tissue inhibitors in human laryngeal carcinoma

Meeting Abstract

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Munich, 16.-20.05.2007. Düsseldorf, Köln: German Medical Science; 2007. Doc07hno088

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2007/07hno088.shtml

Veröffentlicht: 8. August 2007

© 2007 Papadas et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Extensive alterations cartilage extracellular matrix constituents were observed in squamous cell laryngeal carcinoma. These were mainly due to the elevated activity of metalloproteinases, and especially gelatinases, MMP-2 and -9, in unbalance with their specific tissue inhibitors. The work therefore focused in the expression of these macromolecules.

Specimens from 4 healthy individuals and 8 patients who underwent laryngectomy (cancerous and macroscopically normal tissue) were obtained and applied to immunohistochemical and RT-PCR analyses.

The expression of MMP-2 and -9 and of TIMP-1 and -2 was increased in cancer, in a stage-related order. The critical stage was stage II, after which there was not significant elevation in the expressions studied. The immunohistochemical examination suggested that MMP-2 and -9 were expressed mainly from normal cells, TIMP-1 absolutely from normal cells, whereas TIMP-2, especially in late stages, from both normal and cancer cells.

The obtained data demonstrated that tumor progression was closely related to the alteration of the expression of specific extracellular enzymes, the gelatinases, and their relative inhibitors. More specifically, gelatinases were expressed twice as much TIMPs, thus resulting in tumor increase and progression. These changes were easily observed in samples of late stages, suggesting that stage II is the most critical for laryngeal cancer treatment.