gms | German Medical Science

78. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

16.05. - 20.05.2007, München

Effect of erythropoietin on cultured spiral ganglion cells

Meeting Abstract

  • author Nurdanat Berkingali - Hannover Medical School, Hannover, Germany
  • corresponding author Gerrit Paasche - Hannover Medical School, Hannover, Germany
  • Thomas Lenarz - Hannover Medical School, Hannover, Germany
  • Timo Stöver - Hannover Medical School, Hannover, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Munich, 16.-20.05.2007. Düsseldorf, Köln: German Medical Science; 2007. Doc07hno031

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2007/07hno031.shtml

Veröffentlicht: 8. August 2007

© 2007 Berkingali et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Loss of hair cells with consecutive degeneration of spiral ganglion cells (SGC) is the main characteristic of hearing loss. The benefit achieved by the use of cochlear implants depends among other factors on the number of surviving SGC. It has been recently demonstrated that erythropoietin (EPO) attenuates apoptosis of ganglion cells. The aim of the present study is therefore to test the effect of EPO on SGC.

Methods: Spiral ganglion cells were isolated from neonatal rats (p3-5). Dissected and dissociated SGC were cultured for 48 and 72 hours at 37°C and with supplementation of either 2.5 or 5 ng/ml EPO. Surviving SGC, neurite length and cell soma diameter were determined. Results were compared with the effect of brain-derived neurotrophic factor (BDNF) (50 ng/ml). Furthermore, the effect of EPO combined with BDNF was investigated.

Results: The low concentrations of EPO investigated in this study had no effect on SGC. However, in combination with BDNF they further increased survival and neurite length. The mean neurite length of 236 µm (BDNF alone) was increased to 308 µm (BDNF + 5 ng EPO) and 362 µm (BDNF +2.5 ng EPO) after the cultivation period of 48 hours and to 571 nm (BDNF + 5 ng EPO) and 647 µm (BDNF +EPO 2.5 ng/ml) after 72 hours.

Conclusion: Erythropoietin has only a minor protective effect on survival of SGC. However, EPO led to an increased neutrite outgrowth on surviving SGC.