gms | German Medical Science

77. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

24.05. - 28.05.2006, Mannheim

Immunohistological detection of the chemokines IL-8, MCP-1, MIP-1alpha and RANTES - Study of different chemokine antibodies

Immunhistologische Detektierung der Chemokine IL--8, MCP-1, MIP-1alpha und RANTES - Methodenstudie unter Verwendung unterschiedlicher Antikörper

Meeting Abstract

Suche in Medline nach

  • corresponding author presenting/speaker Ulrike Förster - ENT Department, Berlin, Germany
  • Michael Sticherling - Dermatology Department, Leipzig, Germany
  • Heidi Olze - ENT Department, Berlin, Germany

German Society of Otorhinolaryngology, Head and Neck Surgery. 77th Annual Meeting of the German Society of Otorhinolaryngology, Head and Neck Surgery. Mannheim, 24.-28.05.2006. Düsseldorf, Köln: German Medical Science; 2006. Doc06hno001

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2006/06hno001.shtml

Veröffentlicht: 7. September 2006

© 2006 Förster et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Chemokines have recently been described in the pathogenesis of polyposis nasi. However, the immunohistological detection of chemokines is difficult. The aim of the study was to investigate the immunohistological examination. Following specimens of dermatoses were examinated. From in vitro examinations the investigated chemokines were described in the skin.

Methods: Biopsies of patients with psoriasis (n=10), eczema (n=10) and normal skin (n=10) were examined. Cryostat sections were stained with the ABC-kit. Twelve monoclonal and polyclonal antibodies were investigated (IL-8, MCP-1, MIP-1alpha and RANTES). Secondly the chemokine antibodies were tested in a solid phase ELISA.

Results: From 5 IL-8 antibodies one monoclonal antibodiy revealed a specific suprabasal immunoreactivity with less staining according to the grade of inflammation. Another monoclonal IL-8 antibody showed a specific homogeneous reaction in psoriasis, eczema and normal skin. One of 2 tested MIP-1alpha antibodies revealed a specific immunoreactivity in the stratum corneum of normal skin. The other antibodies were negative. The solid phase ELISA revealed predominantly specific reactions.

Conclusions: The immunohistological differences depended on the antibodies. For the characterization of chemokines immunohistological examinations are unreliable and should be complemented through other immunoassays (e.g. ELISA/ PCR).