gms | German Medical Science

76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

04.05. - 08.05.2005, Erfurt

Expression of CD46 und CD150 (SLAM) in normal and otosclerotic temporal bone tissue

Meeting Abstract

  • corresponding author Hans P. Niedermeyer - Dept. of ENT, Technical University of Munich, Germany
  • Tsaagan Gantumur - Dept. of ENT, Technical University of Munich, Germany
  • Irmgard Wiest - Dept. of ENT, Technical University of Munich, Germany
  • Wolfgang J. Neubert - Institue for Virology, Max Planck Institue of Biochemistry, Martinsried
  • Wolfgang Arnold - Dept. of ENT, Technical University of Munich, Germany

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno698

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2005/05hno068.shtml

Veröffentlicht: 22. September 2005

© 2005 Niedermeyer et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Biochemical investigations have shown the presence of measles virus (MeV) type A in the otosclerotic focus. Wildtype MeV infect cells using the CD150 receptor but mutated or attenuated MeV preferentially CD46. The aim of our study was to investigate the pattern of expression of CD46 and CD150 in normal and otosclerotic tissue. We used methanol-acetone fixed cell lines (human osteosarcoma cell line Saos-2, rat osteosarcoma cell line ROS, CHO-cells, all from ATCC, and primary cell culture from otosclerotic and non otosclerotic control tissue form the outer ear canal) such as paraformaldehyde-fixed, EDTA-decalcified otosclerotic bone chips and bone specimen from the outer ear canal. The cell lines and cryostate sections from the bone specimens were investigated with anti-CD46 (Dianova, Hamburg, Germany). Anti-CD150 (Acris, Ebioscience, Hiddenhausen, Germany) was employed on paraffin-embedded tissue section and cell lines. Human and rat osteosarcoma cell lines such as the primary cell cultures from normal and otosclerotic bone expressed strongly CD46 while CHO remained negative. CD150 expression in the cell lines could not be observed. In the tissue sections osteoblasts, osteoclasts, chondrocytes, and epithelial cells expressed CD46, staining for CD150 was only observed in osteoclasts and chondrocytes. Some osteoblasts showed a very weak reaction with CD150. In a previous study we could detect by situ-RT-PCR MeV RNA in osteoclasts, osteoblasts and chondrocytes of the otosclerotic tissue such as the epithelial cells of the middle ear mucosa. Sequencing of the MeV in the otosclerotic tissue revealed the presence of a genotype A (wild type) which infects cells preferentially by CD150. This study has shown the presence of CD150 only on osteoclasts and chondrocytes of normal and otosclerotic bone. Further investigation will elucidate if osteoblasts can be infected by MeV using other receptors.