gms | German Medical Science

7th EFSMA – European Congress of Sports Medicine, 3rd Central European Congress of Physical Medicine and Rehabilitation, Annual Assembly of the German and the Austrian Society of Physical Medicine and Rehabilitation

Austrian Society of Physical Medicine and Rehabilitation

26.-29.10.2011, Salzburg, Österreich

GC-MS analysis of hair for the detection of amphetamines and cocaine and their metabolites. A new modified methodology

Meeting Abstract

  • corresponding author presenting/speaker Konstantinos Natsis - Department of Anatomy, Medical School, Aristotle University, Thessaloniki, Greece
  • author Georgios Psaros - Department of Chemistry, Aristotle University, Thessaloniki, Greece
  • author Alexia Koukou - Department of Chemistry, Aristotle University, Thessaloniki, Greece
  • author Amvrosios Orfanidis - Department of Chemistry, Aristotle University, Thessaloniki, Greece
  • author Orthodoxia Mastrogianni - Laboratory of Forensic Service of Ministry of Justice of Thessaloniki, Thessaloniki, Greece
  • author Georgios Theodoridis - Department of Chemistry, Aristotle University, Thessaloniki, Greece
  • author Heleni Zagelidou - Laboratory of Forensic Service of Ministry of Justice of Thessaloniki, Thessaloniki, Greece
  • author Heleni Tsoukali - Laboratory of Forensic Medicine and Toxicology, Medical School, Aristotle University, Thessaloniki, Greece
  • author Nikolaos Raikos - Laboratory of Forensic Medicine and Toxicology, Medical School, Aristotle University, Thessaloniki, Greece

7th EFSMA – European Congress of Sports Medicine, 3rd Central European Congress of Physical Medicine and Rehabilitation. Salzburg, 26.-29.10.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11esm113

DOI: 10.3205/11esm113, URN: urn:nbn:de:0183-11esm1137

Veröffentlicht: 24. Oktober 2011

© 2011 Natsis et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Hair analysis is recognized as a suitable method for the assessment of long term use of drugs of abuse. In the present paper we describe the development of a new methodology aiming to detect and quantify the presence of amphetamines and cocaine and their metabolites in hair from human subjects including samples of narcotic drug users.

Material/Methods: Hairs were obtained from drug-free humans and were used for method development: All hair samples were washed with water, followed by acetone and finally with dichloromethane. Thirty mg of washed hairs were spiked with the analytes of interest and internal standards, and the sample was subsequently extracted with methanol. Following centrifugation, the extract was evaporated to dryness and BSTFA (1%TMCS) was added to the dry residue. Following derivatisation, analysis was conducted on GC-MS with selected ion monitoring (SIM). The analytes included: cocaine, benzoylecgonine, ecgonine methyl ester, amphetamine, methamphetamine, MDA, MDMA. Internal standards from each category were added (in total three standards).

Results: First the GC-MS (electron impact) analytical method was optimized to reach the best chromatographic separation and highest sensitivity. Three ions were selected for each analyte for SIM and one of these three ions was used for quantitation. Next, the methodology applied for the derivatisation was optimized: derivatisation time, temperature and volume of BSTFA added were studied. The best conditions were 20 min, 70oC and 50 μL of BSTFA respectively. Using the developed methodology, calibration curves were obtained for the 7 analytes (8 concentration points and four independent repetitions for each point). Linearity was found satisfactory (R2 ranged from 0.972 to 0.999). Within day repeatability (RSD less than 15%) was found very satisfactory. Stability of the derivatised sample was found limited to 48 hours. The limit of detection was found in the low ng/mg range (up to 0.5 ng/mg of hairs). The method was successfully applied to the analysis of samples from drug users.

Conclusion: A simple and efficient method was developed and validated for the detection and quantitation of amphetamines and cocaine and their metabolites in trace levels in hair. This tool is complementary to the existing arsenal of analytical tools in the fight against drug abuse, increasing the time span for the detection of drugs of abuse in biological samples.


References

1.
Barroso M, Dias M, Vieira DN, Queiroz JA, Lopez-Rivadulla M. Development and validation of an analytical method for the simultaneous determination of cocaine and its main metabolite, benzoylecgonine, in human hair by gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2008;22:3320-6.
2.
Cordero R, Paterson S. Simultaneous quantification of opiates, amphetamines, cocaine and metabolites and diazepam and metabolite in a single hair sample using GC–MS. Journal of Chromatography B. 2007;850:423-31.
3.
Skender L, Karacic V, Brcic I, Bagaric A. Quantitative determination of amphetamines, cocaine, and opiates in human hair by gas chromatography/mass spectrometry. Forensic Science International. 2002;125:120-6.