Artikel
Detection of mRNA expression of components of the fibrinolytic system in serum-free cultured porcine pigmented ciliary epithelial cells
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Autoren
Veröffentlicht: | 22. September 2004 |
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Gliederung
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Objective
To determine the gene expression encoding for important components of the fibrinolytic system in serum-free cultured pigmented ciliary epithelial cells of the porcine eye.
Methods
Total mRNA of confluent primary cultures of porcine ciliary epithelial cells was isolated after 48 hours of serum-free cultivation. For each component of the fibrinolytic system reverse transcribed mRNA was measured by real time polymerase chain reaction (TaqMan® PCR) with primers and probes deduced from the human genes.
Results
In cultured pigmented ciliary epithelial cells of the porcine eye we detected mRNA expression of plasminogen activator inhibitor-1 (PAI-1), thrombin activatable fibrinolysis inhibitor (TAFI), tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), protein C and protein S.
Conclusions
We localized the gene expression of important components of the fibrinolytic system in cultured porcine pigmented ciliary epithelial cells. PAI-1 and TAFI are main regulators of the fibrinolytic system. Both inhibitors reduce plasminogen activity and attenuate fibrinolysis and proteolysis. In addition, PAI-1 leads to decreased dissolution of extracellular matrix. Therefore, stimulation of PAI-1 and TAFI production may be involved in intraocular inflammation, wound healing processes and perhaps in the pathogenesis of primary open angle glaucoma.
Supported by Deutsche Forschungsgemeinschaft (DFG), ER 259/1