gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Morphological changes of pig corneas after the use of preservatives in an in-vitro cornea penetration model

Meeting Abstract

Suche in Medline nach

  • corresponding author B. Jakopp - Novartis Pharma AG, Ophtha Technical Development, Basel, Schweiz, Universitäts-Augenklinik Basel, Schweiz
  • C. Schoch - Novartis Pharma AG, Ophtha Technical Development, Basel, Schweiz
  • P. Meyer - Universitäts-Augenklinik Basel, Schweiz

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogP 060

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dog2004/04dog551.shtml

Veröffentlicht: 22. September 2004

© 2004 Jakopp et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

With a cornea penetration model using pig eyes, the morphological effect of preservatives on the corneal parenchyma was examined. The goal of this study was to establish this in vitro method.

Methods

Freshly obtained pig corneas were fixed with an O -ring in an Frantz-type-diffusion system. Under physiological conditions (Tears buffer, Aqueous humor buffer, 37°C) the test-solution was applied to the epithelial side (donor chamber) and could now diffuse to the endothelial side (acceptor chamber). Preservatives, such as Benzalkonium chloride (BAC) with a concentration of 0,02 %, Cetrimide 0,02 %, Benzoxonium chloride 0,02 %, and others were tested. They were kept in contact with the cornea for up to 300 minutes. After different contact times (30, 60, 120, 180, 240, 300 minutes) the corneas were sampled and immersed into formalin solution for histological analysis. The pig corneas were rated with a histological score as following: epithelial edema, graduation I - IV, stromal edema, graduation I-II and the condition of the corneal endothelium (intact or damaged).

Results

There was a clear correlation of corneal damage in function of incubation time. The increase in epithelial edema and in stromal edema could be demonstrated for the preservatives in varying degree. BAC showed by far the most pronounced effect on corneal parenchyma. Control eyes incubated with buffer only, did not exhibit these changes. A damaged corneal endothelium was used as a exclusion criterion.

Conclusions

This in vitro model offers the possibility to test and quantify the potentially toxic effects of ophthalmic excipients or complex solutions in a rapid way. Additionally it helps reducing in vivo experiments with rabbits.