gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Cytological examination of biopsies of indeterminate uveal tumours with the ThinPrep method

Meeting Abstract

  • corresponding author R. Boeloeni - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen
  • O. Dirsch - Institut für Pathologie der Universität zu Köln, Köln
  • G. Anastassiou - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen
  • A. Schueler - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen
  • N. Bornfeld - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen
  • H. Schilling - Zentrum für Augenheilkunde, Universitätsklinikum der Universität Duisburg-Essen, Essen

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogFR.11.08

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dog2004/04dog265.shtml

Veröffentlicht: 22. September 2004

© 2004 Boeloeni et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Objective

To evaluate the ThinPrep method as a tool to establish a correct cytological diagnosis in biopsies of indeterminate uveal tumours provided by the vitrectomy cutter.

Methods

26 intraocular biopsies were performed by a three-port pars plana vitrectomy in 25 patients with unclassifiable uveal tumours. One patient had 2 consecutive biopsies on the same eye. The biopsy material underwent cytological examination with the ThinPrep method. In case of enucleation or resection of the tumour the samples also underwent routine histological examination. Additional immunohistochemical stains were performed in selected cases.

Results

In all cases the material was sufficient for further examination. A final diagnosis could be established in 25 out of 26 biopsies (96%). Cytopathologic diagnosis determined the treatment and differed from the initial clinical diagnosis in 3 of 26 cases. 9 eyes (36%) were enucleated and one eye underwent endoresection, so that histological examination could be performed. In 9 cases the cytological diagnosis was confirmed, in one case a metastasis was diagnosed. Postoperative events that were related to the surgery included vitreous hemorrhage in 4 cases, secondary glaucoma in 3 cases and retinal detachment in 2 cases. No late side effects were seen during the mean follow-up time of 6.46 months.

Conclusions

Biopsy of unclear uveal tumours with the vitrectomy cutter is a safe procedure. In the majority of the examined specimens (96%) the result of the cytological examination with the ThinPrep method was informative and determined the further management of the patients.