gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2017)

24.10. - 27.10.2017, Berlin

Evaluate the effect of Taohong Siwu Decoction on repair of steroid-induced osteonecrosis of femoral head in rabbit models by combination of high-resolution MRI and micro-CT

Meeting Abstract

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  • presenting/speaker Guoju Hong - The University of Western Australia, Guangzhou University of Chinese Medicine, Perth, Australia
  • Leilei Chen - Guangzhou University of Chinese Meidicine, Guangzhou, China
  • Xiaorui Han - Guangzhou University of Chinese Meidicine, Guangzhou, China
  • Haibin Wang - Guangzhou University of Chinese Meidicine, Guangzhou, China
  • Wei He - Guangzhou University of Chinese Meidicine, Guangzhou, China

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2017). Berlin, 24.-27.10.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. DocPO30-966

doi: 10.3205/17dkou892, urn:nbn:de:0183-17dkou8921

Veröffentlicht: 23. Oktober 2017

© 2017 Hong et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Objectives: The pathogenesis of steroid-induced osteonecrosis of the femoral head remains unclear. Femoral head reconstruction after collapse is related to the bone remodeling due to disorder of the bone formation-absorption coupling. Taohong Siwu Decoction (TSD) has been shown to have a positive effect on the disease in clinics. However, the mechanisms by which the TSD confers bone repair after osteonecrosis remain poorly understood. To explore the effect of TSD on bone repair in rabbits with steroid-induced femoral head osteonecrosis.

Methods: Total 50 New Zealand rabbits were randomly assigned into three groups: normal control (n=10), model (n=20) and TSD (n=20) groups, respectively. The steroid-induced osteonecrosis of the femoral head animal models were established by intramuscular injection of endotoxin combined with methylprednisolone (MPS). Rabbits in TSDD group were intragastrically administered with 0.3 g/kg of TSD suspension after the last injection of methylprednisolone. The control and model groups were administrated by equal volume of ultrapure water for 8 consecutive weeks. High-resolution MRI, micro-CT and pathological determinations were used to assess the successful models. Protein expression levels of ABCB1, RUNX2, OPN, RANK, RANKL, PPAR, osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) in rabbit femoral heads were detected by western blot assay.

Results and Conclusion: Empty lacunae rate in the model group was significantly different from that in the normal control group. Bone formation was active and kept better trabecular morphology in the TSD group. In the model group, femoral head with a wide range of low-density areas in high-resolution MRI; TSD group showed the relatively normal shape of the femoral head with small areas of low density. Protein expression levels of RUNX2, RANK, RANKL were significantly up-regulated but protein expression levels of ABCB1, OPG, VEGF were significantly down-regulated in the model group compared with the normal control group; whereas, they were all significantly up-regulated in the TSD group compared with the model group, except for RANK and RANKL. The model group, compared with the normal group in the micro-CT, suggested that the value of BV/TV, Conn.D., SMI, Tb.N and Tb.Th were decreased in the model group, while BS/BV, Tb.Sp increased. The values of BS/BV and Tb.Sp were decreased in the medicine group compared with the model one, while Conn.D., SMI, Tb.N increased. These results suggest that the TSD can promote bone repair in steroid-induced femoral head osteonecrosis rabbits by regulating protein expressions of ABCB1, RUNX2, RANK, RANKL, OPG, and VEGF.