gms | German Medical Science

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2014)

28.10. - 31.10.2014, Berlin

Torsion affects the cell viability of nucleus pulpous cells in the intact intervertebral disc

Meeting Abstract

Suche in Medline nach

  • presenting/speaker Samantha Chan - Institut für chirurgische Technologien und Biomechanik, Universitäte Bern, Bern, Switzerland
  • Jochen Walser - Institute für Biomechanik, Zürich, Switzerland
  • Stephen Ferguson - Institute für Biomechanik, Zürich, Switzerland
  • Benjamin Gantenbein - Institut für chirurgische Technologien und Biomechanik, Universitäte Bern, Bern, Switzerland

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2014). Berlin, 28.-31.10.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. DocPO17-569

doi: 10.3205/14dkou673, urn:nbn:de:0183-14dkou6730

Veröffentlicht: 13. Oktober 2014

© 2014 Chan et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: The intervertebral disc continuously experiences different types of repeated dynamic loading, including compression and torsion. Previous studies on the influence of torsion and combined torsion-compression loading revealed a positive effect on the cell viability when a repetitive short-term torsion was applied at a physiological magnitude (±2 degree) to intervertebral disc organ culture [1]. However, after an extended period (8h) of combined torsion-compression loading, substantial cell death was detected in the NP [2]. In this follow-up study, we aimed to investigate the relationship, if any, between the duration of torsion applied to the IVD and the level of NP cell viability.

Methods: Bovine tails were obtained from slaughterhouse within 3 hours of death. Bovine caudal discs with endplates were harvested and supplied with Dulbecco's modified eagle medium (DMEM) and 10% fetal calf serum and cultured in a custom-built multi-axis dynamic loading bioreactor (2). Torsion (±2 degrees) was applied to the samples at a frequency of 0.2 Hz. Torsion was applied for durations of 0, 1, 4 and 8 hours per day, repeated over 7 days. After the last day of loading, disc tissue was dissected for analysis of cell viability and gene expression analysis.

Results and conclusion: Disc NP cell viability remained above 85 % after torsional loading for 0, 1, or 4 hours per day. Viability was statistical significantly reduced to below 70% when torsion was applied for 8 h per day (p=0.03) (Table 1 [Tab. 1]).

The daily duration of torsional loading did not affect the AF cell viability (>80% for all loading durations). The trend of collagen 2 gene up-regulation and MMP 13 down-regulation with an increasing duration of torsion was observed in both NP and AF (Figure 1 [Fig. 1]).

We have demonstrated torsion affect the cell viability of the nucleus pulpous cells that an extended duration (8h) of torsion could inhibit the survival of NP cells within the IVD in organ culture, while 1 to 4 hour of torsion seem not to influence cell viability.

Acknowledgment: Funds from the Orthopedic Department of the Insel University Hospital of Bern and a private donation from Prof. Dr. Paul Heini, Spine Surgeon, Sonnenhof Clinic Bern were received to support this work. This study was performed with the support of the Microscopy Imaging Center (MIC), University of Bern.


References

1.
Chan SC, Ferguson SJ, Wuertz K, Gantenbein-Ritter B. Biological response of the intervertebral disc to repetitive short-term cyclic torsion. Spine. 2011 Nov;36(24):2021-30. DOI: 10.1097/BRS.0b013e318203aea5 Externer Link
2.
Chan SC, Walser J, Käppeli P, Shamsollahi MJ, Ferguson SJ, Gantenbein-Ritter B. Region specific response of intervertebral disc cells to complex dynamic loading: an organ culture study using a dynamic torsion-compression bioreactor. PLoS ONE. 2013;8(8):e72489. DOI: 10.1371/journal.pone.0072489 Externer Link